PXD012644

PXD012644 is an original dataset announced via ProteomeXchange.

Title	Proteomic investigations of human HERC2 mutants: insights into the pathobiology of a neurodevelopmental disorder
Description	HERC2 is a giant protein with E3 ubiquitin ligase activity and other known and suspected functions. Mutations of HERC2 are implicated in the pathogenesis of various cancers and result in various severe neurological conditions in Herc2-mutant mice. Recently, a pleotropic autosomal recessive HERC2-associated syndrome of intellectual disability, autism and variable neurological deficits was described; its pathogenetic basis is largely unknown. Using peripheral blood-derived lymphoblasts from 3 persons with homozygous HERC2 variants and 14 age- and gender-matched controls, we performed unbiased HPLC-tandem mass spectrometry-based proteomic analyses to provide insights into HERC2-mediated pathobiology. We found that out of 3427 detected proteins, there were 812 differentially expressed proteins between HERC2-cases vs. controls. 184 canonical pathways were enriched after FDR adjustment, including mitochondrial function, energy metabolism, EIF2 signaling, immune functions, ubiquitination and DNA repair. Ingenuity Pathway Analysis® identified 209 upstream regulators that could drive the differential expression, prominent amongst which were neurodegeneration-associated proteins. Differentially expressed protein interaction networks highlighted themes of immune function/dysfunction, regulation of cell cycle/cell death, and energy metabolism. Overall, the analysis of the HERC2-associated proteome revealed striking differential protein expression between cases and controls. The large number of differentially expressed proteins likely reflects HERC2’s multiple domains and numerous interacting proteins. Our canonical pathway and protein interaction network findings suggest derangements of multiple pathways in HERC2-associated disease.
HostingRepository	PRIDE
AnnounceDate	2019-03-27
AnnouncementXML	Submission_2019-03-27_03:53:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012644
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kathleen Lundberg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 05:30:40	ID requested
⏵ 1	2019-03-27 03:54:01	announced

Abraham JR, Barnard J, Wang H, Noritz GH, Yeganeh M, Buhas D, Natowicz MR, Proteomic investigations of human HERC2 mutants: Insights into the pathobiology of a neurodevelopmental disorder. Biochem Biophys Res Commun, 512(2):421-427(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, lymphoblast, HERC2, autism, neurodvelopment

Marvin Natowicz
contact affiliation	Pathology and Laboratory Medicine, Genomic Medicine, Neurological and Pediatrics Institutes, Cleveland Clinic
contact email	natowim@ccf.org
lab head
Kathleen Lundberg
contact affiliation	Case Western Reserve University
contact email	kathleen.lundberg@case.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012644
     1. Label: PRIDE project
     2. Name: Proteomic investigations of human HERC2 mutants: insights into the pathobiology of a neurodevelopmental disorder