PXD012635

PXD012635 is an original dataset announced via ProteomeXchange.

Title	An updated perspective on deconvoluting chimeric MS/MS spectra by LC and precursor isolation and their subsequent assignment by the CharmeRT mPSM search strategy
Description	Considering the recent advances in LC and mPSM methodologies, we aimed to provide a systematic comparison and in-depth analysis of the effects that standard and specialized LC setups, as well as different isolation windows, have on the occurrence and assignment of chimeric spectra derived from a complex peptide mixture. To this end, we provide a comprehensive analysis of how these factors influence the chromatographic resolution, isolation interference, and the subsequent identification of MS/MS spectra by a traditional or mPSM algorithm.
HostingRepository	PRIDE
AnnounceDate	2019-05-20
AnnouncementXML	Submission_2019-05-20_08:19:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Abraham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 02:34:43	ID requested
⏵ 1	2019-05-20 08:19:51	announced

Dataset with its publication pending

submitter keyword: tandem mass spectrometry, chimeric spectra, isolation interference, liquid chromatography

Paul Abraham
contact affiliation	Chemical Sciences Division, Oak Ridge National Laboratory
contact email	abrahampe@ornl.gov
lab head
Paul Abraham
contact affiliation	Oak Ridge National Laboratory
contact email	abrahampe@ornl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012635

PRIDE project URI

• PRIDE
  1. PXD012635
     1. Label: PRIDE project
     2. Name: An updated perspective on deconvoluting chimeric MS/MS spectra by LC and precursor isolation and their subsequent assignment by the CharmeRT mPSM search strategy