PXD012632

PXD012632 is an original dataset announced via ProteomeXchange.

Title	STRUCTURAL WEAKENING OF THE COLONIC MUCUS BARRIER IS AN EARLY EVENT IN ULCERATIVE COLITIS PATHOGENESIS
Description	The colonic inner mucus layer protects us from pathogen invasion and commensal-induced inflammation. Mucus abnormalities are common in ulcerative colitis (UC), but their cause and importance are unknown. The aim of this study was to determine the role of compositional mucus barrier alterations in UC. In this single-center case-control study, sigmoid colon biopsies were obtained from UC patients with ongoing inflammation (n=36) and in remission (n=28), and from 47 patients without inflammatory bowel disease. Mucus samples were collected from biopsies ex vivo, and their protein composition analyzed by mass spectrometry. Mucus barrier integrity and goblet cell response to microbial challenge were also assessed for a subset of patients. The core colonic mucus proteome was shown to consist of a small set of 29 secreted proteins. Patients with active UC had reduced levels of major structural components, including the mucin MUC2 (p<0.0001), also in mucus from non-inflamed segments, and their goblet cell secretory response to microbial stimulus was abrogated. Functional mucus barrier failure was observed in a subset of UC patients with and without active inflammation, and accompanied by alterations in proteins associated with mucus secretion and luminal organization. This study represents the first characterization and comparison of the mucus proteomes of the inflamed and non-inflamed colon. Core mucus structural components were reduced in active UC, also in mucus from non-inflamed segments. Thus, weakening of the mucus barrier is likely to occur early in UC pathogenesis, and represents a novel target for intervention.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:26.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	S.S van der Post
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 02:33:19	ID requested
1	2021-03-26 04:38:38	announced
2	2021-03-26 05:19:07	announced	2021-03-26: Updated project metadata.
⏵ 3	2024-10-22 05:20:28	announced	2024-10-22: Updated project metadata.

10.1136/gutjnl-2018-317571;

van der Post S, Jabbar KS, Birchenough G, Arike L, Akhtar N, Sjovall H, Johansson MEV, Hansson GC, Structural weakening of the colonic mucus barrier is an early event in ulcerative colitis pathogenesis. Gut, 68(12):2142-2151(2019) [pubmed]

submitter keyword: LC-MS/MS AND IBD,HUMAN, MUCUS, ILEUM, COLON

Gunnar C Hansson
contact affiliation	Department of Medical Biochemistry, University of Gothenburg
contact email	gunnar.hansson@medkem.gu.se
lab head
S.S van der Post
contact affiliation	Medical Chemistry
contact email	sjoerd.van.der.post@medkem.gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD012632

PRIDE project URI

• PRIDE
  1. PXD012632
     1. Label: PRIDE project
     2. Name: STRUCTURAL WEAKENING OF THE COLONIC MUCUS BARRIER IS AN EARLY EVENT IN ULCERATIVE COLITIS PATHOGENESIS