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PXD012632

PXD012632 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSTRUCTURAL WEAKENING OF THE COLONIC MUCUS BARRIER IS AN EARLY EVENT IN ULCERATIVE COLITIS PATHOGENESIS
DescriptionThe colonic inner mucus layer protects us from pathogen invasion and commensal-induced inflammation. Mucus abnormalities are common in ulcerative colitis (UC), but their cause and importance are unknown. The aim of this study was to determine the role of compositional mucus barrier alterations in UC. In this single-center case-control study, sigmoid colon biopsies were obtained from UC patients with ongoing inflammation (n=36) and in remission (n=28), and from 47 patients without inflammatory bowel disease. Mucus samples were collected from biopsies ex vivo, and their protein composition analyzed by mass spectrometry. Mucus barrier integrity and goblet cell response to microbial challenge were also assessed for a subset of patients. The core colonic mucus proteome was shown to consist of a small set of 29 secreted proteins. Patients with active UC had reduced levels of major structural components, including the mucin MUC2 (p<0.0001), also in mucus from non-inflamed segments, and their goblet cell secretory response to microbial stimulus was abrogated. Functional mucus barrier failure was observed in a subset of UC patients with and without active inflammation, and accompanied by alterations in proteins associated with mucus secretion and luminal organization. This study represents the first characterization and comparison of the mucus proteomes of the inflamed and non-inflamed colon. Core mucus structural components were reduced in active UC, also in mucus from non-inflamed segments. Thus, weakening of the mucus barrier is likely to occur early in UC pathogenesis, and represents a novel target for intervention.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:20:26.960.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterS.S van der Post
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-08 02:33:19ID requested
12021-03-26 04:38:38announced
22021-03-26 05:19:07announced2021-03-26: Updated project metadata.
32024-10-22 05:20:28announced2024-10-22: Updated project metadata.
Publication List
10.1136/gutjnl-2018-317571;
van der Post S, Jabbar KS, Birchenough G, Arike L, Akhtar N, Sjovall H, Johansson MEV, Hansson GC, Structural weakening of the colonic mucus barrier is an early event in ulcerative colitis pathogenesis. Gut, 68(12):2142-2151(2019) [pubmed]
Keyword List
submitter keyword: LC-MS/MS AND IBD,HUMAN, MUCUS, ILEUM, COLON
Contact List
Gunnar C Hansson
contact affiliationDepartment of Medical Biochemistry, University of Gothenburg
contact emailgunnar.hansson@medkem.gu.se
lab head
S.S van der Post
contact affiliationMedical Chemistry
contact emailsjoerd.van.der.post@medkem.gu.se
dataset submitter
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Dataset FTP location
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