PXD012626

PXD012626 is an original dataset announced via ProteomeXchange.

Title	Macrostomum bioadhesion
Description	The flatworm Macrostomum lignano features a duo-gland adhesive system that allows it to repeatedly attach to and release from substrates in seawater within a minute. However, little is known about the molecules involved in this temporary adhesion. In this study, we showed that the attachment of M. lignano relieds on the secretion of two large adhesive proteins, Macrostomum lignano adhesion protein 1 (Mlig-ap1) and Mlig-ap2. We revealed that both proteins are expressed in the adhesive gland cells and that their distribution within the adhesive footprints was spatially restricted. RNA interference knock-down experiments demonstrated the essential function of these two proteins in flatworm adhesion. Negatively-charged modified sugars in the surrounding water inhibited flatworm attachment, while positively-charged molecules impeded detachment. In addition, we found that M. lignano could not adhere to strongly hydrated surfaces. We propose an attachment-release model where Mlig-ap2 attaches to the substrate and Mlig-ap1 exhibits a cohesive function. A small negatively-charged molecule is secreted that interferes with Mlig-ap1, inducing detachment. These findings are of relevance for fundamental adhesion science and efforts to mitigate biofouling. Further, this first model of flatworm temporary adhesion may serve as the starting point for the development of synthetic reversible adhesion systems for medicinal and industrial applications.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-24_23:57:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Ladurner
SpeciesList	scientific name: Macrostomum lignano; NCBI TaxID: 282301;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-08 01:16:04	ID requested
1	2019-02-15 06:44:01	announced
⏵ 2	2019-02-24 23:57:23	announced	Updated publication reference for PubMed record(s): 30782790.

Wunderer J, Lengerer B, Pjeta R, Bertemes P, Kremser L, Lindner H, Ederth T, Hess MW, Stock D, Salvenmoser W, Ladurner P, A mechanism for temporary bioadhesion. Proc Natl Acad Sci U S A, 116(10):4297-4306(2019) [pubmed]

curator keyword: Biological

submitter keyword: flatworms, bioadhesion, Platyhelminthes, bioadhesives, detattchment

Peter Ladurner
contact affiliation	Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, 6020 Innsbruck, Austria
contact email	peter.ladurner@uibk.ac.at
lab head
Peter Ladurner
contact affiliation	University of Innsbruck
contact email	peter.ladurner@uibk.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012626

PRIDE project URI

• PRIDE
  1. PXD012626
     1. Label: PRIDE project
     2. Name: Macrostomum bioadhesion