PXD012622

PXD012622 is an original dataset announced via ProteomeXchange.

Title	Interspecies comparison of the bacterial response to allicin reveals species-specific defense strategies
Description	Allicin, a volatile diallylthiosulfinate from garlic (Allium sativum), exhibits broad-spectrum activity against microbial pathogens. It disrupts thiol and redox homeostasis, proteostasis, and cell membrane integrity leading to inactivation of even multidrug resistant strains. Since medicine demands cost-efficient antimicrobials with so far unexploited mechanisms, allicin with its multifaceted mode of action is a promising lead structure for future therapeutics. However, while progress is being made in fully unraveling its mode of action, little is known on bacterial adaptation strategies to cope with allicin-like stress. Some isolates of Pseudomonas aeruginosa and Escherichia coli, withstand exposure to higher allicin concentrations than other bacteria due to as yet unknown cellular mechanisms. To elucidate resistance and sensitivity-conferring cellular processes we compared the acute proteomic responses of the resistant species P. aeruginosa and sensitive species Bacillus subtilis to the published proteomic response of E. coli to allicin treatment. The cellular defense strategies shared functional features: proteins involved in protein (re)folding and repair, ROS and RSS detoxification, and cell envelope modification were upregulated. In both Gram-negative species, protein synthesis of up to 64% of the proteins synthesized in untreated cells was down-regulated while the sensitive, Gram-positive B. subtilis responded by upregulation of multiple regulons. A comparison of the B. subtilis proteomic response to a library of proteomic responses to antibiotic treatment, revealed 30 proteins specifically upregulated by allicin. Other upregulated proteins indicating oxidative stress were shared with nitrofurantoin and diamide. Microscopy-based assays further indicate that in B. subtilis cell wall integrity was impaired.
HostingRepository	PRIDE
AnnounceDate	2021-03-09
AnnouncementXML	Submission_2021-03-09_05:23:50.548.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christoph Senges
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-07 23:56:49	ID requested
1	2021-03-09 02:47:50	announced
⏵ 2	2021-03-09 05:23:51	announced	2021-03-09: Updated publication reference for PubMed record(s): 31622046.

W, ü, llner D, Haupt A, Prochnow P, Leontiev R, Slusarenko AJ, Bandow JE, Interspecies Comparison of the Bacterial Response to Allicin Reveals Species-Specific Defense Strategies. Proteomics, 19(24):e1900064(2019) [pubmed]

curator keyword: Biological

submitter keyword: allicin, mode of action, proteomic response, Bacillus subtilis, Pseudomonas aeruginosa

Julia E. Bandow
contact affiliation	Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany
contact email	julia.bandow@rub.de
lab head
Christoph Senges
contact affiliation	Ruhr University Bochum
contact email	christoph.senges@rub.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012622
     1. Label: PRIDE project
     2. Name: Interspecies comparison of the bacterial response to allicin reveals species-specific defense strategies