PXD012617

PXD012617 is an original dataset announced via ProteomeXchange.

Title	Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A
Description	Peroxiredoxins are modulators of aging in yeast and multicellular organisms. The mechanisms by which peroxiredoxins stimulate H2O2 resistance and slow down aging are, however, unclear. Here we show that the yeast peroxiredoxin Tsa1 boosts H2O2 resistance and prevents aging independent of cellular H2O2 levels, but in a manner dependent on H2O2 signaling. More specifically, we pin-point a role of Tsa1 in repressing nutrient signaling via protein kinase A (PKA) that governs both H2O2 resistance and longevity. Tsa1 controls PKA activity at the level of the catalytic subunits and Tpk1 is redox-modified by Tsa1 on a conserved cysteine residue (Cys243) in Tpk1 upon H2O2 addition boosting cellular H2O2 resistance. Tpk1 redox modification dephosphorylates a conserved threonine 241 in the activation loop reducing enzyme activity. We discuss these results in the context of an integrative view on aging where nutrient signaling pathways constitute hubs integrating information from multiple aging-related conduits.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:38.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); iodoacetamide derivatized residue; L-cysteine glutathione disulfide
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-07 04:50:33	ID requested
1	2020-07-22 05:52:40	announced
⏵ 2	2023-11-14 08:51:44	announced	2023-11-14: Updated project metadata.

curator keyword: Biological

submitter keyword: PRM, Yeast, oxidative stress,Saccharomyces cerevisiae, LC-MS/MS

Mikael Molin
contact affiliation	Department of Chemistry and Molecular Biology, University of Gothenburg, Sweden
contact email	mikael.molin@cmb.gu.se
lab head
Wolfgang Reiter
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012617
     1. Label: PRIDE project
     2. Name: Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of protein kinase A