PXD012611

PXD012611 is an original dataset announced via ProteomeXchange.

Title	Inefficient secretion of anti-sigma factor FlgM inhibits bacterial motility at high temperature
Description	Regulation of gene expression according to the environmental conditions is vitally important for all living organisms. Temperature is one of the key cues for microorganisms that can exist in different environments, and several mechanisms of temperature-dependent re-allocation of cellular resources have been described. Here we compared transcriptome and proteome of E. coli grown at basal (37°C) and febrile temperatures (42°C) of the mammalian host. We demonstrated that the downregulation of Escherichia coli motility at high temperature primarily occurred due to the malfunction of secretion apparatus, apparently related to the disassembly of the flagellar basal body. For proteome analysis we performed data-independent acquisition (DIA) mass spectrometry via Spectronaut. The Spectronaut file can be assessed with the freely available Spectronaut viewer (https://www.biognosys.com/technology/spectronaut-viewer).
HostingRepository	PRIDE
AnnounceDate	2019-08-14
AnnouncementXML	Submission_2019-08-14_05:23:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-07 03:53:09	ID requested
⏵ 1	2019-08-14 05:23:07	announced

Rudenko I, Ni B, Glatter T, Sourjik V, Inefficient Secretion of Anti-sigma Factor FlgM Inhibits Bacterial Motility at High Temperature. iScience, 16():145-154(2019) [pubmed]

curator keyword: Biological

submitter keyword: Data-independent acquisition, Motolity, E.coli

Victor Sourjik
contact affiliation	Dep Systems and Syntheic Microbiology Max Plank Institute for terrestrial Microbiology Marburg, Germany
contact email	victor.sourjik@synmikro.mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012611
     1. Label: PRIDE project
     2. Name: Inefficient secretion of anti-sigma factor FlgM inhibits bacterial motility at high temperature