PXD012608

PXD012608 is an original dataset announced via ProteomeXchange.

Title	A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs
Description	Gcn5 and sirtuins are conserved HAT and HDAC enzymes that were first characterised as regulators of gene expression via histone acetylation. Although histone tails are important substrates of these enzymes, they also target non-histone proteins in diverse processes. Previously, we used SILAC-based mass spectrometry to identify novel non-histone substrates of Gcn5 and sirtuins in yeast. We found a shared target consensus sequence. In our latest work we used a synthetic biology approach to demonstrate that this consensus sequence can direct acetylation and deacetylation targeting by these enzymes in vivo. We used the synthetic substrate as a tool to prioritize SILAC-based acetylome analyses of SAGA mutants. Presented here are analyses of ada3∆ mutants analyzed in this work versus wild-type control. The strains and labels used are described in the BioRxiv report linked in the methods sections below.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-03-04_02:31:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Downey
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-07 03:43:23	ID requested
1	2019-02-24 23:55:59	announced
⏵ 2	2019-03-04 02:31:23	announced	Updated publication reference for PubMed record(s): 30804216.

R, ö, ssl A, Denoncourt A, Lin MS, Downey M, A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs. J Biol Chem, 294(16):6227-6239(2019) [pubmed]

curator keyword: Biological

submitter keyword: Gcn5, acetylation, sirtuin, SAGA, Ada3, consensus sequence, synthetic biology

Michael Downey
contact affiliation	Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada Ottawa Institute of Systems Biology, Ottawa, ON, Canada
contact email	mdowne2@uottawa.ca
lab head
Michael Downey
contact affiliation	University of Ottawa
contact email	mdowne2@uottawa.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012608

PRIDE project URI

• PRIDE
  1. PXD012608
     1. Label: PRIDE project
     2. Name: A synthetic non-histone substrate to study substrate targeting by the Gcn5 HAT and sirtuin HDACs