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PXD012604

PXD012604 is an original dataset announced via ProteomeXchange.

Dataset Summary

  • Title: A dataset describing a suite of novel antibody reagents for the RAS signaling network
  • Description: RAS genes are frequently mutated in cancer and have for decades eluded effective therapeutic attack. The National Cancer Institute‚Äôs RAS Initiative has a focus on understanding pathways and discovering therapies for RAS-driven cancers. Part of these efforts is the generation of novel reagents to enable the quantification of RAS network proteins. Here we present a dataset describing the development, validation (following consensus principles developed by the broader research community), and distribution of 104 monoclonal antibodies (mAbs) enabling detection of 27 phosphopeptides and 69 unmodified peptides from 20 proteins in the RAS network. The dataset characterizes the utility of the antibodies in a variety of applications, including Western blotting, immunoprecipitation, protein array, immunohistochemistry, and targeted mass spectrometry. All antibodies and characterization data are publicly available through the CPTAC Antibody Portal, Panorama Public Repository, and/or PRIDE databases. These reagents will aid researchers in discerning pathways and measuring expression changes in the RAS signaling network.
  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2019-09-03
  • AnnouncementXML: Submission_2019-09-03_10:03:11.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original dataset
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Regine Schoenherr
  • SpeciesList: scientific name: Homo sapiens; NCBI TaxID: 9606;
  • ModificationList: Carbamidomethyl; Phospho; Label:13C(6)15N(2); Label:13C(3)15N(1); Label:13C(6)15N(4); Label:13C(6)15N(1); Label:13C(5)15N(1)
  • Instrument: QTRAP 5500; QTRAP 6500

Dataset History

RevisionDatetimeStatusChangeLog Entry
02019-02-06 21:58:03ID requested
12019-08-13 15:38:55announced
22019-09-03 10:03:13announcedAdded publication

Publication List

  1. Schoenherr RM, Huang D, Voytovich UJ, Ivey RG, Kennedy JJ, Saul RG, Colantonio S, Roberts RR, Knotts JG, Kaczmarczyk JA, Perry C, Hewitt SM, Bocik W, Whiteley GR, Hiltke T, Boja ES, Rodriguez H, Whiteaker JR, Paulovich AG, A dataset describing a suite of novel antibody reagents for the RAS signaling network. Sci Data, 6(1):160(2019) [pubmed]

Keyword List

  1. submitter keyword: RAS proteins, peptide-based immuno-MRM, monoclonal antibodies

Contact List

    Amanda G Paulovich
    • contact affiliation: Fred Hutchinson Cancer Research Center
    • contact email: apaulovi@fredhutch.org
    • lab head:
    Regine Schoenherr
    • contact affiliation: Fred Hutchinson Cancer Research Center
    • contact email: rschoenh@fhcrc.org
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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