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PXD012592

PXD012592 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemical crosslinking and mass spectrometry of SumoE1, Sumo and Fat10
DescriptionIn this study, the proteins SumoE1 and Fat10 or Fat10-AV mutant were crosslinked using the crosslinking reagent H12/D12 BS3 in order to determine specific interaction sites of Fat10 and SumoE1. Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-XL-MS) was used to compare crosslink abundances of SumoE1 in presence of Sumo as well as Sumo and Fat10 versus crosslink abundances of SumoE1 in absence of Sumo and Fat10 in order to determine the influence of Sumo and Fat10 on the structural dynamics of SumoE1.
HostingRepositoryPRIDE
AnnounceDate2019-10-02
AnnouncementXMLSubmission_2019-10-07_02:39:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian Stengel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-01 06:13:11ID requested
12019-10-02 01:23:43announced
22019-10-07 02:39:35announced2019-10-07: Updated publication reference for PubMed record(s): 31575873.
Publication List
Aichem A, Sailer C, Ryu S, Catone N, Stankovic-Valentin N, Schmidtke G, Melchior F, Stengel F, Groettrup M, The ubiquitin-like modifier FAT10 interferes with SUMO activation. Nat Commun, 10(1):4452(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Fat10, SumoE1, XL-MS, q-XL-MS
Contact List
Florian Stengel
contact affiliationDepartment of Biology, University of Konstanz, Germany
contact emailflorian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliationUniversität Konstanz
contact emailflorian.stengel@uni-konstanz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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