PXD012592 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemical crosslinking and mass spectrometry of SumoE1, Sumo and Fat10 |
Description | In this study, the proteins SumoE1 and Fat10 or Fat10-AV mutant were crosslinked using the crosslinking reagent H12/D12 BS3 in order to determine specific interaction sites of Fat10 and SumoE1. Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-XL-MS) was used to compare crosslink abundances of SumoE1 in presence of Sumo as well as Sumo and Fat10 versus crosslink abundances of SumoE1 in absence of Sumo and Fat10 in order to determine the influence of Sumo and Fat10 on the structural dynamics of SumoE1. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-02 |
AnnouncementXML | Submission_2019-10-07_02:39:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Stengel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-01 06:13:11 | ID requested | |
1 | 2019-10-02 01:23:43 | announced | |
⏵ 2 | 2019-10-07 02:39:35 | announced | 2019-10-07: Updated publication reference for PubMed record(s): 31575873. |
Publication List
Aichem A, Sailer C, Ryu S, Catone N, Stankovic-Valentin N, Schmidtke G, Melchior F, Stengel F, Groettrup M, The ubiquitin-like modifier FAT10 interferes with SUMO activation. Nat Commun, 10(1):4452(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Fat10, SumoE1, XL-MS, q-XL-MS |
Contact List
Florian Stengel |
contact affiliation | Department of Biology, University of Konstanz, Germany |
contact email | florian.stengel@uni-konstanz.de |
lab head | |
Florian Stengel |
contact affiliation | Universität Konstanz |
contact email | florian.stengel@uni-konstanz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD012592 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012592
- Label: PRIDE project
- Name: Chemical crosslinking and mass spectrometry of SumoE1, Sumo and Fat10