PXD012591

PXD012591 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics
Description	In this study, we evaluated three well-established sample preparation methods for shotgun proteomics, named: i) UREA; ii) sodium dodecyl sulfate (SDS); and iii) filter-aided sample preparation (FASP) protocols. We focused our analysis on protein extraction and digestion from heart left ventricle (LV) tissue.The three methods were compared by relative quantification using dimethyl labeling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:58.696.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Verano-Braga
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	isotope labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-01 05:55:57	ID requested
1	2019-05-21 00:36:25	announced
⏵ 2	2024-10-22 04:53:03	announced	2024-10-22: Updated project metadata.

Publication List

Rodrigues-Ribeiro L, Melo-Braga MN, Kjeldsen F, G, ó, mez-Mendoza DP, Verano-Braga T, Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics. Anal Biochem, 578():51-59(2019) [pubmed]
10.1016/j.ab.2019.05.006;

Rodrigues-Ribeiro L, Melo-Braga MN, Kjeldsen F, G, ó, mez-Mendoza DP, Verano-Braga T, Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics. Anal Biochem, 578():51-59(2019) [pubmed]

10.1016/j.ab.2019.05.006;

Keyword List

ProteomeXchange project tag: Human Proteome Project
curator keyword: Biological
submitter keyword: LC-MSMS,Rat, dimethyl labeling, Heart, FASP

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biological

submitter keyword: LC-MSMS,Rat, dimethyl labeling, Heart, FASP

Contact List

Thiago Verano-Braga
contact affiliation	Departament of Physiology and Biophysics, Federal University of Minas Gerais
contact email	tvbraga@icb.ufmg.br
lab head
Thiago Verano-Braga
contact affiliation	Federal University of Minas Gerais
contact email	npf.ufmg@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012591
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012591

PRIDE project URI

Repository Record List

[ + ]