PXD012584

PXD012584 is an original dataset announced via ProteomeXchange.

Title	Secretome and intracellular proteome of eccE1 and mycP1 deletion mutants of Mycobacterium tuberculosis
Description	Mycobacterium tuberculosis uses the specialised ESX-1 secretion system to secrete virulence factors and potent immunogenic effectors required for disease progression. ESX-1 is a multi-subunit apparatus with a membrane complex that is predicted to form a pore in the cytoplasmic membrane. In M. tuberculosis this complex is composed of five membrane proteins: EccB1, EccCa1, EccCb1, EccD1, EccE1. In this study, we have characterised the membrane component EccE1, and we found that deletion of eccE1 leads to destabilisation of EccB1, EccCa1 and EccD1 levels as well as to the disruption of EsxA, EsxB, EspA and EspC secretion. Surprisingly, secretion of EspB was not affected by loss of EccE1.
HostingRepository	PRIDE
AnnounceDate	2019-12-16
AnnouncementXML	Submission_2019-12-16_02:14:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrej Benjak
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-01 01:42:20	ID requested
⏵ 1	2019-12-16 02:14:02	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Mycobacterium tuberculosis, ESX-1, eccE1, mycP1

Stewart Thomas Cole
contact affiliation	Global Health Institute, EPFL, Lausanne, Switzerland
contact email	stewart.cole@epfl.ch
lab head
Andrej Benjak
contact affiliation	EPFL
contact email	abenjak@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012584
     1. Label: PRIDE project
     2. Name: Secretome and intracellular proteome of eccE1 and mycP1 deletion mutants of Mycobacterium tuberculosis