PXD012582

PXD012582 is an original dataset announced via ProteomeXchange.

Title	Indoxyl sulfate and p-cresyl sulfate directly promote vascular calcification via activation of inflammation and coagulation pathways
Description	Vascular calcification contributes to high cardiovascular mortality in chronic kidney disease (CKD) patients. An association between the uremic toxins indoxyl sulfate (IS) and p-cresyl sulfate (PCS) and cardiovascular disease has been suggested. This study provides strong etiological evidence for indoxyl sulfate and p-cresyl sulfate as major contributors to vascular calcification in chronic kidney disease patients. Continuous exposure to indoxyl sulfate or p-cresyl sulfate in rats with chronic kidney disease promotes moderate to severe calcification in the aorta and peripheral vessels. Unbiased proteomic analyses of arterial samples coupled to functional bioinformatics annotation analysis revealed that calcification events were associated with acute phase response signaling, coagulation and glucometabolic signaling pathways, while escape from toxin-induced calcification was linked with liver X receptors and farnesoid X/liver X receptor signaling pathways. Activation of inflammation and coagulation pathways in the arterial wall plays a pivotal role in toxin-induced calcification and strongly associates with hyperglycemia and insulin resistance. These findings reveal new perspectives to establish novel therapeutic targets to prevent, halt progression or cure vascular calcification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:43.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abdelkrim Azmi
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-01 01:16:07	ID requested
1	2019-06-12 18:19:44	announced
⏵ 2	2024-10-22 04:52:47	announced	2024-10-22: Updated project metadata.

Opdebeeck B, Maudsley S, Azmi A, De Mar, é A, De Leger W, Meijers B, Verhulst A, Evenepoel P, D'Haese PC, Neven E, Indoxyl Sulfate and p-Cresyl Sulfate Promote Vascular Calcification and Associate with Glucose Intolerance. J Am Soc Nephrol, 30(5):751-766(2019) [pubmed]

10.1681/asn.2018060609;

ProteomeXchange project tag: Kidney Urine (B/D-HPP), Cardiovascular (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP)

curator keyword: Biomedical

submitter keyword: indoxyl sulfate,chronic kidney disease, vascular calcification, inflammation, hyperglycemia, coagulation, p-cresyl sulfate, iTRAQ-8plex

Patrick C. D’Haese
contact affiliation	Labo Pathophysiology, Department of Biomedical Sciences, University of Antwerp. Belgium
contact email	Patrick.dhaese@uantwerpen.be
lab head
Abdelkrim Azmi
contact affiliation	VIB-UAntwerp Center for Molecular Neurology
contact email	Abdelkrim.Azmi@uantwerpen.vib.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012582

PRIDE project URI

• PRIDE
  1. PXD012582
     1. Label: PRIDE project
     2. Name: Indoxyl sulfate and p-cresyl sulfate directly promote vascular calcification via activation of inflammation and coagulation pathways