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DataSet Summary

  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2019-08-13
  • AnnouncementXML: Submission_2019-08-13_16:09:56.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Yaamini Venkataraman
  • Title: Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences
  • Description: Global climate change is rapidly altering coastal marine ecosystems important for food production. A comprehensive understanding of how organisms will respond to these complex environmental changes can come only from observing and studying species within their natural environment. To this end, the effects of environmental drivers — pH, dissolved oxygen content, salinity, and temperature — on Pacific oyster (Crassostrea gigas) physiology were evaluated in an outplant experiment. Sibling juvenile oysters were outplanted to eelgrass and unvegetated habitat at five different estuarine sites within the Acidification Nearshore Monitoring Network in Washington State, USA to evaluate how regional environmental drivers influence molecular physiology. Within each site, we also determined if eelgrass presence that buffered pH conditions changed the oysters’ expressed proteome. A novel, two-step, gel-free proteomic approach was used to identify differences in protein abundance in C. gigas ctenidia tissue after a 29 day outplant by 1) identifying proteins in a data independent acquisition survey step and 2) comparing relative quantities of targeted environmental response proteins using selected reaction monitoring. While there was no difference in protein abundance detected between habitats or among sites within Puget Sound, C. gigas outplanted at Willapa Bay had significantly higher abundances of antioxidant enzymes and molecular chaperones. Environmental factors at Willapa Bay, such as higher average temperature, may have driven this protein abundance pattern. These findings generate a suite of new hypotheses for lab and field experiments to compare the effects of regional conditions on physiological responses of marine invertebrates.
  • SpeciesList: scientific name: Crassostrea gigas; NCBI TaxID: 29159;
  • ModificationList: Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
  • Instrument: Orbitrap Fusion Lumos; TSQ Vantage

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-01-30 13:31:57ID requested
12019-08-13 16:09:57announced

Publication List

  1. Venkataraman YR, Timmins-Schiffman E, Horwith MJ, Lowe AT, Nunn B, Vadopalas B, Spencer LH, Roberts SB. Characterization of Pacific oyster Crassostrea gigas proteomic response to natural environmental differences. Marine Ecology Progress Series. 2019 Feb 1;610:65-81. DOI: https://doi.org/10.3354/meps12858

Keyword List

  1. submitter keyword: proteomics, oysters, in situ measurements, estuarine systems, antioxidant enzymes, molecular chaperones

Contact List

    Steven Roberts
    • contact affiliation: University of Washington, School of Aquatic and Fishery Sciences
    • contact email: sr320@uw.edu
    • lab head:
    Yaamini Venkataraman
    • contact affiliation: University of Washington School of Aquatic and Fishery Sciences
    • contact email: yaaminiv@uw.edu
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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