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PXD012564

PXD012564 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpecific inhibition of splicing factor activity by decoy RNA oligonucleotides
DescriptionAlternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. We designed decoy oligonucleotides, composed of several repeats of a RNA motif, which is recognized by a single SF. Here we show that decoy oligonucleotides targeting splicing factors RBFOX1/2, SRSF1 and PTBP1, can specifically bind to their respective SFs and inhibit their splicing and biological activities both in vitro and in vivo. These decoy oligonucleotides present a novel approach to specifically downregulate SF activity and have the potential to treat diseases where SFs are up-regulated, such as cancer.
HostingRepositoryPRIDE
AnnounceDate2019-01-31
AnnouncementXMLSubmission_2019-05-27_00:09:36.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTamar Geiger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-30 07:41:08ID requested
12019-01-31 05:16:23announced
22019-05-27 00:09:38announcedUpdated publication reference for PubMed record(s): 30962446.
Publication List
Denichenko P, Mogilevsky M, Cléry A, Welte T, Biran J, Shimshon O, Barnabas GD, Danan-Gotthold M, Kumar S, Yavin E, Levanon EY, Allain FH, Geiger T, Levkowitz G, Karni R, Specific inhibition of splicing factor activity by decoy RNA oligonucleotides. Nat Commun, 10(1):1590(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Alternative Splicing, Splicing factors, RBFOX2, PTBP1, SRSF1, Cancer, Decoy oligonucleotides, lc-ms
Contact List
Tamar Geiger
contact affiliationDepartment of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
contact emailgeiger@tauex.tau.ac.il
lab head
Tamar Geiger
contact affiliationTel Aviv University
contact emailgeiger@tauex.tau.ac.il
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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