PXD012563

PXD012563 is an original dataset announced via ProteomeXchange.

Title	USP8 maintains ESC stemness via deubiquitination of EPG5
Description	The coiled-coil domain of EPG5 was synthesized as follows: Biotin-MNKNEAVSQQIHVLQKEVRQLQAE AAQ (from Beijing SciLight Biotechnology, LCC). 1 mg of this short peptide was incubated with ES cells lysates followed by immunoprecipitation with anti-biotin beads. The precipitates were extensively washed with lysis buffer then boiled with SDS loading buffer and subjected to SDS–PAGE. Total proteins were excised from the gels, then subjected to in-gel digestion and analysis by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-05-27_01:09:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xingxing shi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-30 07:19:34	ID requested
1	2019-02-24 23:49:45	announced
⏵ 2	2019-05-27 01:09:25	announced	Updated publication reference for PubMed record(s): 30931944.

Gu H, Shi X, Liu C, Wang C, Sui N, Zhao Y, Gong J, Wang F, Zhang H, Li W, Zhao T, USP8 maintains embryonic stem cell stemness via deubiquitination of EPG5. Nat Commun, 10(1):1465(2019) [pubmed]

ProteomeXchange project tag: Stems Cells (B/D-HPP)

curator keyword: Technical, Biological

submitter keyword: i.e.mouse.escs.epg5.usp8.LC-MSMS

i.e.Tongbiao Zhao
contact affiliation	Professor of Stem Cell and Immunology State Key Laboratory of Reproductive Biology Institute of Zoology, Chinese Academy of Sciences Address:1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China
contact email	tbzhao@ioz.ac.cn
lab head
xingxing shi
contact affiliation	INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
contact email	shixingixng@yeah.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012563

PRIDE project URI

• PRIDE
  1. PXD012563
     1. Label: PRIDE project
     2. Name: USP8 maintains ESC stemness via deubiquitination of EPG5