PXD012561

PXD012561 is an original dataset announced via ProteomeXchange.

Title	Progressive Changes in Human Follicular Fluid Composition over the Course of Ovulation: Quantitative Proteomic Analyses.
Description	The composition of follicular fluid reflects crucial paracrine signalling between granulosa- and theca cells and the oocyte. Dynamic changes in the protein composition of human follicular fluid across multiple time points in the period where final meiotic maturation and release of the oocyte take place are previously undescribed. Twenty-five women undergoing IVF- or ICSI treatment in a standard antagonist protocol with agonist ovulation trigger were included in this prospective cohort study. From each patient one follicle was aspirated by transvaginal ultrasound guided puncture at one of five specific time points either before ovulation induction (T=0) or 12-, 17-, 32- or 36 hours after ovulation induction (five patients/time point). Proteomics was carried out by liquid chromatography-mass spectrometry. In total, 400 proteins were identified (FDR<0.05) and 30 were significantly regulated across time points (one-way ANOVA, adjusted p<0.05). Compared to compiled human plasma proteome sets, 47 proteins were unique to follicular fluid. Enriched biological functions among differentially expressed proteins included immune functions, wound healing and functions related to blood coagulation (FDR<0.02). The most profound changes occurred shortly after ovulation induction. We confirmed parallel protein expression to known granulosa cell mRNA changes and described many hitherto unknown protein expression profiles during ovulation. Thus, the study endorsed important biological functions of some proteins and suggested additional proteins, which may be crucial to intrafollicular signalling and oocyte competence that should be further investigated.
HostingRepository	PRIDE
AnnounceDate	2019-07-30
AnnouncementXML	Submission_2019-08-01_04:42:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Indira Pla Parada
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-30 02:48:37	ID requested
1	2019-07-29 03:59:30	announced
2	2019-07-30 00:30:18	announced	Updated project metadata.
⏵ 3	2019-08-01 04:43:00	announced	Updated project metadata.

Poulsen LC, Pla I, Sanchez A, Gr, ø, ndahl ML, Marko-Varga G, Yding Andersen C, Englund ALM, Malm J, Progressive changes in human follicular fluid composition over the course of ovulation: quantitative proteomic analyses. Mol Cell Endocrinol, 495():110522(2019) [pubmed]

submitter keyword: Follicular fluid, proteome, ovulation, oocyte maturation, human

Johan Malm
contact affiliation	Deputy head of department at Department of Translational Medicine. Lund University. Principal investigator at CEBMMS PI. Professor at Clinical Chemistry, Malmö Professor at Clinical Protein Science and Imaging Professor at Department of Clinical Sciences, Lund
contact email	johan.malm@med.lu.se
lab head
Indira Pla Parada
contact affiliation	Lund university
contact email	indira.pla_parada@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012561

PRIDE project URI

• PRIDE
  1. PXD012561
     1. Label: PRIDE project
     2. Name: Progressive Changes in Human Follicular Fluid Composition over the Course of Ovulation: Quantitative Proteomic Analyses.