PXD012551

PXD012551 is an original dataset announced via ProteomeXchange.

Title	Intricate structure of the interphase chromocenter revealed by the analysis of a factor involved in species formation
Description	In higher eukaryotes centromeres often coalesce into a large intranuclear domain called the chromocenter. Chromocenters are important for the organization of pericentric heterochromatin and a disturbance of their formation results in an upregulation of repetitive elements and causes defects in chromosome segregation. Mutations in the gene encoding for the centromere associated Drosophila speciation factor HMR show very similar phenotypes suggesting a role of HMR in chromocenter architecture and function. We performed confocal and super resolution microscopy as well as proximity based biotinylation experiments of HMR, centromeric protein dCenpA and heterochromatic protein HP1a to generate a molecular map of HMR, dCenpA and HP1a bound chromatin. Our work reveals an intricate internal structure of the centromeric chromatin region, which suggests a role of HMR in separating heterochromatin from centromeric chromatin.
HostingRepository	PRIDE
AnnounceDate	2020-03-23
AnnouncementXML	Submission_2020-03-23_00:08:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-29 02:56:35	ID requested
⏵ 1	2020-03-23 00:08:10	announced

Kochanova NY, Schauer T, Mathias GP, Lukacs A, Schmidt A, Flatley A, Schepers A, Thomae AW, Imhof A, A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation. Nucleic Acids Res, 48(8):4161-4178(2020) [pubmed]

curator keyword: Biological

submitter keyword: Drosophila, chromatin proteomics, centromeres, chromocenter

Axel Imhof
contact affiliation	Chromatin Proteomics Biomedical Center, LMU München Grosshaderner Strasse 9 D-82152 Planegg-Martinsried +4989218075420
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	Biomedical Center, LMU
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012551

PRIDE project URI

• PRIDE
  1. PXD012551
     1. Label: PRIDE project
     2. Name: Intricate structure of the interphase chromocenter revealed by the analysis of a factor involved in species formation