PXD012550

PXD012550 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of three cell populations from the common starfish Asterias rubens
Description	The origin of cells involved in regeneration in Echinoderms is an open question till now. Most experimental data support the process of transdifferentiation as the main mechanism, although the activity of progenitor/stem cells is also considered. The renewal of coelomocytes, the cells which occupy the coelomic cavity in Asterias rubens is an example of physiological regeneration. Although it is considered that coelomic epithelium is the main source of coelomocytes, many details of this process remain unclear. In this study, we provide evidence of the origin of several types of coelomocytes in A. rubens from the coelomic epithelium, with particular emphasis on small undifferentiated cells. The concept of a pool of marginal coelomocytes is introduced as cells sequestered on the surface of the coelomic epithelium and characterized by a special cellular composition. Proteomic analysis by LC-MALDI TOF/TOF MS identified in total 403 proteins in coelomocytes (COE), coelomic epithelium (CE) and coelomic epithelium subpopulation enriched with undifferentiated cells (CEW). Proteins common and associated with each cell pool were revealed and the intermediate status of cells on the border of coelomic epithelium and coelom was confirmed. The potential players involved in regenerative processes were identified.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:10:06.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Shabelnikov
SpeciesList	scientific name: Asterias rubens; NCBI TaxID: 7604;
ModificationList	iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-29 02:02:21	ID requested
⏵ 1	2024-10-08 11:10:11	announced

Sharlaimova N, Shabelnikov S, Bobkov D, Martynova M, Bystrova O, Petukhova O, Coelomocyte replenishment in adult Asterias rubens: the possible ways. Cell Tissue Res, 383(3):1043-1060(2021) [pubmed]

10.1007/s00441-020-03337-z;

curator keyword: Biological

submitter keyword: coelomic epithelium, progenitor cells,Asterias rubens, LC-MALDI, coelomocytes, SCIEX 5800

Sergey Shabelnikov
contact affiliation	Laboratory of Regulation of Gene Expression, Institute of Cytology, Russian Academy of Sciences
contact email	sergey.v.shabelnikov@gmail.com
lab head
Sergey Shabelnikov
contact affiliation	Institute if Cytology of RAS
contact email	sergey.v.shabelnikov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD012550

PRIDE project URI

• PRIDE
  1. PXD012550
     1. Label: PRIDE project
     2. Name: Proteome profiling of three cell populations from the common starfish Asterias rubens