PXD012548

PXD012548 is an original dataset announced via ProteomeXchange.

Title	Akt1 depletion in murine ovarian granulosa cells reveals known and novel molecular and cellular processes dependent on this kinase
Description	The PI3K/AKT signaling pathway is known to regulate a broad range of cellular processes and it is often altered in several types of cancers. Recently, somatic AKT1 mutations leading to a strong activation of this kinase have been reported in juvenile granulosa cell tumors. However, the molecular role of AKT1 in the ovary is still poorly understood. To get insights into its ovarian function, we depleted Akt1 in murine primary granulosa cells, a supporting cell lineage, and assessed the molecular consequences at both the transcript and protein levels. We were able to corroborate the involvement of AKT1 in metabolism, apoptosis, cell cycle or cytoskeleton regulation in granulosa cells. Consistently, we showed that established granulosa cells depleted for Akt1 exhibited increased velocity and altered directional persistent migration. This study also allowed us to uncover new direct and indirect targets of the kinase. Indeed, a series of proteins involved in intracellular transport and mitochondrial physiology were also significantly affected by Akt1 depletion. Using in silico analyses, we also propose a set of kinases and transcription factors that can mediate the action of AKT1 on the deregulated transcripts and proteins. Taken altogether, our results provide a resource of direct and indirect AKT1 targets in the ovary and may shed light on the molecular events driving tumorigenesis owing to its mutations in granulosa cells.
HostingRepository	PRIDE
AnnounceDate	2019-04-30
AnnouncementXML	Submission_2019-05-02_05:48:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-29 02:01:56	ID requested
1	2019-04-30 06:01:07	announced
⏵ 2	2019-05-02 06:08:29	announced	Updated publication reference for PubMed record(s): 30992313.

Elzaiat M, Herman L, Legois B, L, é, ger T, Todeschini AL, Veitia RA, High-throughput Exploration of the Network Dependent on AKT1 in Mouse Ovarian Granulosa Cells. Mol Cell Proteomics, 18(7):1307-1319(2019) [pubmed]

curator keyword: Biological

submitter keyword: AKT1, granulosa cells, mouse, Knock-Down, transcriptomics, proteomics

Reiner A. VEITIA
contact affiliation	Professor of Genetics, PrCEx2 Université Paris-Diderot/Paris 7. Team: Molecular Oncology and Ovarian Pathologies Institut Jacques Monod, 15 Rue Hélène Brion, Paris Cedex 13, France.
contact email	reiner.veitia@ijm.fr
lab head
Thibaut LEGER
contact affiliation	Institut Jacques Monod
contact email	thibaut.leger@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012548
     1. Label: PRIDE project
     2. Name: Akt1 depletion in murine ovarian granulosa cells reveals known and novel molecular and cellular processes dependent on this kinase