PXD012531

PXD012531 is an original dataset announced via ProteomeXchange.

Title	The yeast AIF homolog Nde1 integrates metabolic and proteostatic signals on the mitochondrial surface and executes cell death
Description	Little is known about the proteolytic turnover of mitochondrial proteins under static growth conditions. Combining dynamic isotope labeling and mass spectrometry in yeast cells, we found an exceptionally high turnover for the NADH dehydrogenase Nde1. This homolog of the mammalian apoptosis inducing factor AIF forms two distinct topomers in mitochondria, one residing in the intermembrane space and one cytosol-exposed form that spans the outer membrane. The latter serves as pro-apoptotic factortrigger of cell death with the potential to kill yeast cells in response pro-apoptotic stimuli. The surface-exposed topomer is strongly enriched in respiratory deficient cells and its turnover is executed cooperatively by the cytosolic proteasome and the mitochondrial protease Yme1. Our data suggest that in addition to their role in electron transfer, mitochondrial NADH dehydrogenases such as Nde1 or AIF integrate signals from energy metabolism and cytosolic proteostasis to eliminate compromised individuals from growing cell populations.
HostingRepository	PRIDE
AnnounceDate	2019-11-06
AnnouncementXML	Submission_2019-11-06_01:42:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Boos
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-28 01:53:36	ID requested
1	2019-11-06 00:58:38	announced
⏵ 2	2019-11-06 01:42:12	announced	2019-11-06: Updated publication reference for PubMed record(s): 31668496.

Saladi S, Boos F, Poglitsch M, Meyer H, Sommer F, M, ü, hlhaus T, Schroda M, Schuldiner M, Madeo F, Herrmann JM, The NADH Dehydrogenase Nde1 Executes Cell Death after Integrating Signals from Metabolism and Proteostasis on the Mitochondrial Surface. Mol Cell, 77(1):189-202.e6(2020) [pubmed]

curator keyword: Biological

submitter keyword: Protein Turnover, Yeast, Mitochondria, Apoptosis, Nde1

Johannes M. Herrmann
contact affiliation	Univeristy of Kaiserslautern Cell Biology
contact email	hannes.herrmann@bio.uni-kl.de
lab head
Felix Boos
contact affiliation	University of Kaiserslautern
contact email	fboos@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012531

PRIDE project URI

• PRIDE
  1. PXD012531
     1. Label: PRIDE project
     2. Name: The yeast AIF homolog Nde1 integrates metabolic and proteostatic signals on the mitochondrial surface and executes cell death