PXD012526

PXD012526 is an original dataset announced via ProteomeXchange.

Title	Global quantification of intact proteins via chemical isotope labeling and mass spectrometry
Description	Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2,283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved into the important biological processes such as stress response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:10.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheyi Liu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-28 01:44:55	ID requested
1	2019-04-30 11:53:34	announced
2	2019-05-02 04:19:16	announced	Updated publication reference for PubMed record(s): 30990045.
3	2019-05-02 06:30:47	announced	Updated publication reference for PubMed record(s): 30990045.
⏵ 4	2024-10-22 04:49:16	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00071;

Liu Z, Wang R, Liu J, Sun R, Wang F, Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry. J Proteome Res, 18(5):2185-2194(2019) [pubmed]

curator keyword: Biological

submitter keyword: Intact protein quantification, pTOP, Chemical isotope labeling, Highest isotopic peak,Mass spectrometry

Fangjun Wang
contact affiliation	CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China
contact email	wangfj@dicp.ac.cn
lab head
Zheyi Liu
contact affiliation	Doctor
contact email	zy_liu@dicp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012526

PRIDE project URI

• PRIDE
  1. PXD012526
     1. Label: PRIDE project
     2. Name: Global quantification of intact proteins via chemical isotope labeling and mass spectrometry