PXD012525

PXD012525 is an original dataset announced via ProteomeXchange.

Title	Schistosomes- exosomal miRNAs: modulators of T helper (CD+) cell differentiation
Description	Schistosomiasis (Bilharziasis) is caused by the helminth Schistosoma. The acute stage of the infection extends from the penetration of the parasite through the host skin until it reaches its final location as adult female and male in copula, at the blood vessels around the gut or bladder. The female starts then depositing fertile eggs. The chronic stage of schistosomiasis starts with laying the eggs which promote a strong immunological Th2 response. It is unclear, so far, how this Th2 response gradually declines even though the parasitic worms live for years and continue to produce eggs. Here we demonstrate that schistosomes downregulate the differentiation toward the Th2 lineage by modulating the Th2 transcriptional program. Moreover, we found that adult schistosomes secrete micro-vesicles, possessing exosomal characteristics, and containing miRNAs, which have been found in Th2 cells that exposed indirectly to schistosomes. Importantly, we demonstrate that, the schistosoma miR-10 molecule targets MAP3K7, which consequently down modulates NF-kB. Since NF-kB is a critical factor necessary for Th2 differentiation and function, our results can at least partially explain, the decrease of the Th2 response over time of infection This exosomal worm-host immune cell interaction, can be further utilized for the development of novel diagnostic and therapeutic approaches to schistosomiasis.
HostingRepository	PRIDE
AnnounceDate	2019-12-10
AnnouncementXML	Submission_2020-01-15_03:49:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-28 01:22:33	ID requested
1	2019-12-10 02:31:22	announced
⏵ 2	2020-01-15 03:49:25	announced	2020-01-15: Updated publication reference for PubMed record(s): 31825165.

Meningher T, Barsheshet Y, Ofir-Birin Y, Gold D, Brant B, Dekel E, Sidi Y, Schwartz E, Regev-Rudzki N, Avni O, Avni D, Schistosomal extracellular vesicle-enclosed miRNAs modulate host T helper cell differentiation. EMBO Rep, 21(1):e47882(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Schistosomes, T helper, miRNA

Dror Avni
contact affiliation	Laboratory of Molecular Cell Biology, Center for Cancer Research and Department of Medicine C, Molecular Laboratory for the Study of Tropical Diseases,Sheba Medical Center, Tel Hashomer.
contact email	droravni@msn.com
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD012525

PRIDE project URI

• PRIDE
  1. PXD012525
     1. Label: PRIDE project
     2. Name: Schistosomes- exosomal miRNAs: modulators of T helper (CD+) cell differentiation