PXD012523

PXD012523 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of glioblastoma cells
Description	PKR-like kinase (PERK) plays a significant role in inducing angiogenesis in various cancer-types including glioblastoma. Mass spectrometry screen on conditioned media from LN308 glioblastoma cell line treated with hypoxia revealed Peptidylglycine α-amidating monooxygenase (PAM) as a potential target inducing angiogenesis. PERK is found to be regulating PAM at mRNA level. PERK activation via CCT020312 (PERK activator) also increased the cleavage and thus the generation of sfCD domain of PAM in an oxygen-independent manner which acts as a signaling molecule from cytoplasm to the nuclei. PERK was found interacting with PAM in immuno-precipitation experiments suggesting a possible involvement in the generation of the PAM sfCD domain. Knockdown of PERK or PAM in LN308 cells reduced the formation of tubes by HUVECs in vitro. In vivo data also highlighted the importance of PAM in growth of glioblastoma where reduction of PAM expression in engrafted tumor significantly increased the survival in mice. In summary, PAM acts as a potential target for therapy against angiogenesis in glioblastoma.
HostingRepository	PRIDE
AnnounceDate	2020-03-05
AnnouncementXML	Submission_2020-03-05_00:10:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012523
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chi Nguyen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-28 01:20:52	ID requested
⏵ 1	2020-03-05 00:25:24	announced

Soni H, Bode J, Nguyen CDL, Puccio L, Ne, ß, ling M, Piro RM, Bub J, Phillips E, Ahrends R, Eipper BA, Tews B, Goidts V, -amidating monooxygenase supports angiogenesis in glioblastoma. Oncogenesis, 9(2):18(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: secretome, label-free, DDA, shot-gun proteomics, glioblastoma, PERK inhibitor

Robert Ahrends
contact affiliation	Dr. Robert Ahrends Group leader lipidomics Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany
contact email	robert.ahrends@isas.de
lab head
Chi Nguyen
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Bunsen-Kirchhoff-Straße 11 | 44139 Dortmund, Germany
contact email	chi.nguyen@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012523

PRIDE project URI

• PRIDE
  1. PXD012523
     1. Label: PRIDE project
     2. Name: Secretome analysis of glioblastoma cells