PXD012513

PXD012513 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Lactobacillus nagelii in the presence of Saccharomyces cerevisiae isolated from water kefir and comparison with Lactobacilus hordei
Description	Water kefir is a slightly alcoholic and traditionally fermented beverage, which is prepared from sucrose, water, kefir grains, and dried or fresh fruits (e.g. figs). Lactobacillus (L.) nagelii, L. hordei and Saccharomyces (S.) cerevisiae are predominant and stable lactic acid bacteria and yeasts, respectively, isolated from water kefir consortia. The growth of L. nagelii and L. hordei are improved in the presence of S. cerevisiae. In this work we demonstrate that quantitative comparative proteomics enables the investigation of interactions between LAB and yeast to predict real-time metabolic exchange in water kefir. It revealed 73 differentially expressed (DE) in L. nagelii TMW 1.1827 in the presence of S. cerevisiae. The presence of the yeast induced changes in the changes in the carbohydrate metabolism of L. nagelii and affected reactions involved in NAD+/NADH homeostasis. Furthermore, the DE enzymes involved in amino acid biosynthesis or catabolism predict that S. cerevisiae releases glutamine, histidine, methionine and arginine, which are subsequently used by L. nagelii to ensure its survival in the water kefir consortium. In co-culture with S. cerevisiae, L. nagelii profits from riboflavin, most likely secreted by the yeast. The reaction of L. nagelii to the presence of S. cerevisiae differs from that one of the previously studied L. hordei, which displays 233 differentially expressed proteins, changes in citrate metabolism and an antidromic strategy for NAD+/NADH homeostasis. So far, aggregation promotion factors, i.e. formation of a specific glucan and bifunctional enzymes were only detected in L. hordei.
HostingRepository	PRIDE
AnnounceDate	2019-02-28
AnnouncementXML	Submission_2019-03-26_05:47:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Lactobacillus nagelii; NCBI TaxID: 82688; scientific name: Lactobacillus hordei; NCBI TaxID: 468911; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-25 07:19:27	ID requested
1	2019-02-28 04:03:54	announced
⏵ 2	2019-03-26 05:47:06	announced	Updated publication reference for PubMed record(s): 30891008.

Bechtner J, Xu D, Behr J, Ludwig C, Vogel RF, . Front Microbiol, 10():325(2019) [pubmed]

curator keyword: Biological

submitter keyword: Lactobacillus nagelii, Lactobacillus hordei, functional genome prediction, proteomic analysis, metabolism

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technische Universität München (TUM) Gregor-Mendel-Straße 4 85354 Freising DEUTSCHLAND / GERMANY Tel. / Phone: +49 8161 71-6130 E-Mail: tina.ludwig@tum.de www.baybioms.tum.de
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012513
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Lactobacillus nagelii in the presence of Saccharomyces cerevisiae isolated from water kefir and comparison with Lactobacilus hordei