PXD012506

PXD012506 is an original dataset announced via ProteomeXchange.

Title	An integrated global analysis of compartmentalized HRAS signaling - Phosphoproteomics
Description	Modern omics technologies allow us obtaining global information on different types of biological networks. However, integrating these different types of analyzes into a coherent framework for a comprehensive biological interpretation remains challenging. Here, we present a conceptual framework that integrates protein interaction, phosphoproteomics and transcriptomics data. Applying this method to analyze HRAS signaling from different subcellular compartments shows that spatially-defined networks contribute specific functions to HRAS signaling. Changes in HRAS protein interactions at different sites lead to different kinase activation patterns that differentially regulate gene transcription. HRAS mediated signaling is the strongest from the plasma membrane, but it regulates the largest number of genes from the endoplasmic reticulum. The integrated networks provide a topologically and functionally resolved view of HRAS signaling. They reveal new HRAS functions including the control of cell migration from the endoplasmic reticulum and p53 dependent cell survival when signaling from the Golgi apparatus.
HostingRepository	PRIDE
AnnounceDate	2019-03-08
AnnouncementXML	Submission_2019-03-18_07:04:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Matallanas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-25 02:08:27	ID requested
1	2019-03-08 00:25:56	announced
⏵ 2	2019-03-18 07:04:21	announced	Updated publication reference for PubMed record(s): 30865897.

Santra T, Herrero A, Rodriguez J, von Kriegsheim A, Iglesias-Martinez LF, Schwarzl T, Higgins D, Aye TT, Heck AJR, Calvo F, Agudo-Ib, á, ñ, ez L, Crespo P, Matallanas D, Kolch W, An Integrated Global Analysis of Compartmentalized HRAS Signaling. Cell Rep, 26(11):3100-3115.e7(2019) [pubmed]

curator keyword: Biological

submitter keyword: RAS phosphoproteomics MiNETEi localisation

David Gomez
contact affiliation	Systems Biology Ireland, University College Dublin
contact email	david.gomez@ucd.ie
lab head
David Matallanas
contact affiliation	Systems Biology Ireland, University College Dublin
contact email	david.gomez@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012506

PRIDE project URI

• PRIDE
  1. PXD012506
     1. Label: PRIDE project
     2. Name: An integrated global analysis of compartmentalized HRAS signaling - Phosphoproteomics