PXD012503

PXD012503 is an original dataset announced via ProteomeXchange.

Title	Haemonchus contortus secretome LC-MS/MS
Description	The excretory-secretory (ES) proteins are the molecules that actively or passively released by parasite at the parasite-host interface to maintain a physiological and immunological relationship with the host. In the present study, we characterised the excretory-secretory products from the short-term in vitro culture (i.e. within 12 h) of Haemonchus contortus (as the most important parasitic nematodes of livestock animals worldwide) using a high throughput tandem mass-spectrometry underpinned by the most recent genomic dataset. Totally, 878 unique ES proteins from three key developmental stages were identified and quantified without intracellular protein contamination (e.g. histone) typically occurred in longer term maintenance cultures. Bioinformatic analyses showed noteworthy protein excretion and secretion alterations during the transition from the free-living to the parasitic phase, especially for ES proteins which are likely involved in nutrient digestion, acquisition and parasite-host interactions, such as proteolytic cascade related peptidases, glycoside hydrolases, C-type lectins, transthyretin-like proteins and SCP/TAPS proteins. Our finding provide an avenue to better explore interactive processes between the host and this highly significant parasitic nematode, to underpin the search for novel drug and vaccine targets.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:03.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Wang
SpeciesList	scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-25 01:38:29	ID requested
1	2019-06-18 09:03:16	announced
2	2019-07-15 01:07:53	announced	Updated publication reference for PubMed record(s): 31071474.
⏵ 3	2023-11-14 08:50:05	announced	2023-11-14: Updated project metadata.

Wang T, Ma G, Ang CS, Korhonen PK, Koehler AV, Young ND, Nie S, Williamson NA, Gasser RB, High throughput LC-MS/MS-based proteomic analysis of excretory-secretory products from short-term in vitro culture of Haemonchus contortus. J Proteomics, 204():103375(2019) [pubmed]

curator keyword: Metaproteomics, Biological

submitter keyword: Haemonchus contortus,LC-MS/MS, Parasitic nematode, Secretome

Robin Beat Gasser
contact affiliation	Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria 3010, Australia
contact email	robinbg@unimelb.edu.au
lab head
Tao Wang
contact affiliation	The University of Melbourne
contact email	tao.wang1@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012503
     1. Label: PRIDE project
     2. Name: Haemonchus contortus secretome LC-MS/MS