PXD012501

PXD012501 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Thylakoid protein phosphorylation in Physcomitrella patens
Description	Physcomitrella patens is a model plant representing the group of mosses, which diverged from vascular plant ancestors early after land colonization. To investigate the unique characteristic of the composition and post-translational regulation of Physcomitrella patens photosynthetic apparatus, we have separated the thylakoids protein complexes in their subunits composition by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE), followed by protein in-gel tryptic digestion of single spots. To identify the kinase responsible for phosphorylation of the major thylakoid protein complex photosystem II, a mutant line lacking the ortholog of kinase STN8 in the model angiosperm Arabidopsis thaliana has been generated and the identified thylakoid protein phosphorylation pattern have been compared with the WT moss.
HostingRepository	PRIDE
AnnounceDate	2019-05-08
AnnouncementXML	Submission_2019-05-08_04:33:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012501
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Trotta
SpeciesList	scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218;
ModificationList	Phospho; Oxidation; MappingA; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-25 01:25:16	ID requested
⏵ 1	2019-05-08 04:33:20	announced

Publication List

Gerotto C, Trotta A, Bajwa AA, Mancini I, Morosinotto T, Aro EM, Thylakoid Protein Phosphorylation Dynamics in a Moss Mutant Lacking SERINE/THREONINE PROTEIN KINASE STN8. Plant Physiol, 180(3):1582-1597(2019) [pubmed]

Gerotto C, Trotta A, Bajwa AA, Mancini I, Morosinotto T, Aro EM, Thylakoid Protein Phosphorylation Dynamics in a Moss Mutant Lacking SERINE/THREONINE PROTEIN KINASE STN8. Plant Physiol, 180(3):1582-1597(2019) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: Physcomitrella patens
moss
thylakoids
photosynthesis
photosystem II
STN8 kinase
protein phosphorylation
N-terminal acetylation
nLC-MSMS

curator keyword: Biological

submitter keyword: Physcomitrella patens

moss

thylakoids

photosynthesis

photosystem II

STN8 kinase

protein phosphorylation

N-terminal acetylation

nLC-MSMS

Contact List

Eva-Mari Aro
contact affiliation	Molecular Plant Biology, Department of Biochemistry, University of Turku
contact email	evaaro@utu.fi
lab head
Andrea Trotta
contact affiliation	Molecular Plant Biology, Department of Biochemistry, University of Turku
contact email	andrea.trotta@utu.fi
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD012501

PRIDE project URI

Repository Record List

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