PXD012499

PXD012499 is an original dataset announced via ProteomeXchange.

Title	Candidate proteins involved in the calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) identified by iTRAQ analysis
Description	‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) is an important market pear in China. The shape and quality of the fruit is negatively affected by the presence of a persistent calyx. Here, to explore the molecular mechanism of calyx abscission, we designed an experiment to compare protein expression at two critical stages of the calyx abscission process under three treatments: a calyx abscising treatment (6000 × Flusilazole + 300 × PBO), a calyx persisting treatment (50 mg L−1 GA3), and a water control. We investigated the collected protein fragments using isobaric tags for relative and absolute protein quantitation (iTRAQ) to identify candidate proteins and perform relative quantification. We identified 378,078 spectra and 3,873 proteins, of which there were 2,371 differentially abundant proteins (DAPs) having Gene Ontology terms and associating with 124 defined pathways from the Kyoto Encyclopedia of Genes and Genomes. The DAPs that were correlated with calyx abscission were mainly those known to be involved in photosynthesis, plant hormone signal transduction, cell-wall modification, and carbohydrate metabolism. Quantitative real-time PCR was used to confirm the results of the digital transcript abundance measurements. Among the isolated candidate proteins, polygalacturonase and chitinase appear to play key roles during the process of calyx abscission. We identified candidate proteins that exhibit highly dynamic expression changes during the calyx abscission progress. These proteins are potential targets for future functional identification and should be valuable to explore the mechanism of the calyx abscission, and finally for the development of a method for inducing calyx abscission in fruit production based on the use of small molecules.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:22.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Gong
SpeciesList	scientific name: Pyrus x bretschneideri; NCBI TaxID: 225117;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-25 01:23:54	ID requested
1	2020-06-16 22:50:17	announced
⏵ 2	2024-10-22 05:07:27	announced	2024-10-22: Updated project metadata.

Qi X, Wu J, Wang L, Li L, Cao Y, Tian L, Dong X, Zhang S, Identifying the candidate genes involved in the calyx abscission process of 'Kuerlexiangli' (Pyrus sinkiangensis Yu) by digital transcript abundance measurements. BMC Genomics, 14(1):727(2013) [pubmed]

10.1186/1471-2164-14-727;

curator keyword: Biological

submitter keyword: ITRAQ,Pear, Calyx abscission, Differentially abundant proteins

Shutian Tao
contact affiliation	College of Horticulture, Nanjing Agricultural University, China
contact email	taost@njau.edu.cn
lab head
Xin Gong
contact affiliation	College of Horticulture, Nanjing Agricultural University
contact email	1293046895@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012499
     1. Label: PRIDE project
     2. Name: Candidate proteins involved in the calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) identified by iTRAQ analysis