PXD012495

PXD012495 is an original dataset announced via ProteomeXchange.

Title	Characterization and discrimination of Tibetan and Duroc × (Landrace × Yorkshire) pork using label-free quantitative proteomics analysis
Description	The main aim of this study was to unravel key proteins for the differentiation of Tibetan (n=15) and Duroc × (Landrace × Yorkshire) (n=15) pork. A platform consisting of LC-MS/MS analysis and label-free quantitative proteomics was utilized. Changes in the proteome profile were observed for different pork cuts. A total of 91 and 116 differentially expressed proteins (fold change >2 or <0.5, p-value<0.05) were identified in the five cuts (shoulder, rump, loin, shank and belly) of Tibetan (TP) and Duroc × (Landrace × Yorkshire) (DLY) pork, respectively. Meanwhile, a comparative proteomic analysis was performed between the TP and DLY pork. We identified 102 altered expressed proteins, of which 52.9% (n=54) and 47.1% (n=48) were up- and down-regulated, respectively, in DLY pork compared to TP. Functional analysis of these proteins revealed that the most significantly enriched gene ontology term for biological process was purine-containing compound metabolic process (p=0.003), while that for molecular function was threonine-type peptidase activity (p=0.002), and that for cellular component was mitochondrial inner membrane (p=0.001). The most significantly enriched KEGG pathway was involved in histidine metabolism (p=0.01), followed by oxidative phosphorylation (p=0.02). Using chemometrics approach, we identify 68 significant proteins for the discrimination of TP and DLY pork. The most significantly upregulated proteins in TP and DLY pork were nicotinamide nucleotide transhydrogenase and heat shock protein 90-beta, respectively. This study demonstrates the feasibility of using differential proteomic analysis to discriminate between TP and DLY pork and the current data set can be expanded to a larger sample size for possible discriminant validation.
HostingRepository	iProX
AnnounceDate	2019-01-24
AnnouncementXML	Submission_2019-12-10_22:11:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Si Mi
SpeciesList	scientific name: Sus scrofa domesticus; NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-24 08:44:49	ID requested
1	2019-01-24 08:49:17	announced
⏵ 2	2019-12-10 22:11:50	announced	2019-12-11: Update publication information.

Mi S, Li X, Zhang CH, Liu JQ, Huang DQ, Yorkshire) pork using label-free quantitative proteomics analysis. Food Res Int, 119():426-435(2019) [pubmed]

submitter keyword: Characterization, Discrimination, Pork, Meat cuts, Proteomics, LC-MS/MS, Chemometrics

Chun-Hui Zhang
contact affiliation	Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences
contact email	zhangchunhui@caas.cn
lab head
Si Mi
contact affiliation	Institute of Food Science and Technology
contact email	misi@caas.cn
dataset submitter

iProX dataset URI