PXD012486

PXD012486 is an original dataset announced via ProteomeXchange.

Title	Network rewiring of homologous recombination during mitotic proliferation and meiosis
Description	Homologous recombination is essential for high-fidelity DNA repair during mitotic proliferation and meiosis. Yet, context-specific modifications must tailor the recombination machinery to avoid, or enforce, formation of reciprocal exchanges – crossovers – between recombining chromosomes. To obtain molecular insight into how crossover control is achieved, we affinity-purified the 7 DNA-processing enzymes with known roles in channelling HR intermediates into crossovers, or non-crossovers, from vegetative cells, or cells undergoing meiosis. Using mass spectrometry, we provide the first global characterization of their composition. The resulting mitosis- and meiosis-specific interaction maps reveal intricate changes in enzyme architecture and a concerted rewiring of the interaction networks to support flexible control to the recombination outcome. Moreover, functional analyses of 31 novel interactions uncovered 8 meiosis-specific network components that remodel HR to support crossing-over. Chd1, which transiently associates with Exo1 during meiosis, enables the formation of MutLγ-Exo1-dependent crossovers through its conserved ability to bind and displace nucleosomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:15:37.462.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilaria Piazza
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-24 00:31:52	ID requested
1	2019-07-31 04:54:39	announced
2	2019-08-01 04:43:28	announced	Updated publication reference for PubMed record(s): 31351878.
⏵ 3	2024-10-22 04:15:38	announced	2024-10-22: Updated project metadata.

10.1016/j.molcel.2019.06.022;

Wild P, Susperregui A, Piazza I, D, ö, rig C, Oke A, Arter M, Yamaguchi M, Hilditch AT, Vuina K, Chan KC, Gromova T, Haber JE, Fung JC, Picotti P, Matos J, Network Rewiring of Homologous Recombination Enzymes during Mitotic Proliferation and Meiosis. Mol Cell, 75(4):859-874.e4(2019) [pubmed]

submitter keyword: crossing-over, Yen1/GEN1, Mlh1-Mlh3-Exo1, Sgs1/BLM-Top3-Rmi1, Slx1-Slx4(BTDB12), Mus81-Mms4/EME1, Srs2/RTEL1, cell cycle, Mph1/FANCM, Holliday junction,DNA repair

Paola Picotti
contact affiliation	ETH Zurich
contact email	paola.picotti@bc.biol.ethz.ch
lab head
Ilaria Piazza
contact affiliation	MDC Berlin
contact email	ilaria.piazza@bc.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012486

PRIDE project URI

• PRIDE
  1. PXD012486
     1. Label: PRIDE project
     2. Name: Network rewiring of homologous recombination during mitotic proliferation and meiosis