PXD012484 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta |
Description | Marine sponges represent one of the few eukaryotic groups that ubiquitously harbor symbiotic members of the Thaumarchaeota, which are important chemoautotrophic ammonia-oxidizers in many environments. However in most studies, direct demonstration of ammonia-oxidation by these archaea within sponges is lacking, and little is known about sponge-specific adaptations of archaeal ammonia oxidizers (AOA). In this study, we characterized the thaumarchaeal symbiont of the marine sponge Ianthella basta using metaproteogenomics, fluorescence in situ hybridization, qPCR and direct isotope-based functional assays. We demonstrate that the I. basta symbiont is not closely related to other genomically sequenced sponge AOA and is a member of a new genus. “Candidatus Nitrosospongia bastadiensis” is an abundant symbiont that is solely responsible for nitrite formation from ammonia in I. basta that surprisingly does not harbor nitrite-oxidizing microbes. Consistently, Ca N. bastadiensis encodes and expresses the genetic repertoire required for chemolithoautotrophic ammonia oxidation. Furthermore, we show that this AOA is equipped with an expanded set of extracellular subtilisin-like proteases, a metalloprotease unique among archaea, as well as a putative branched-chain amino acid ABC transporter. This repertoire is strongly indicative of a mixotrophic lifestyle and is (with slight variations) also found in other sponge-associated, but not in free-living AOA. We predict that this feature as well as an expanded and unique set of secreted serpins (protease inhibitors), a unique array of eukaryotic-like proteins, and a DNA-phosporothioation system likely involved in defense against foreign DNA, represent important adaptations of AOA to life within these ancient filter-feeding animals. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-13 |
AnnouncementXML | Submission_2019-11-12_18:19:15.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD012484 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Stephanie Markert |
SpeciesList | scientific name: Archaea; NCBI TaxID: 2157; scientific name: Ianthella basta; NCBI TaxID: 375146; scientific name: Bacteria; NCBI TaxID: 2; |
ModificationList | Oxidation |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-23 23:50:59 | ID requested | |
⏵ 1 | 2019-11-12 18:19:16 | announced | |
Publication List
Moeller FU, Webster NS, Herbold CW, Behnam F, Domman D, Albertsen M, Mooshammer M, Markert S, Turaev D, Becher D, Rattei T, Schweder T, Richter A, Watzka M, Nielsen PH, Wagner M, Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta. Environ Microbiol, 21(10):3831-3854(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Sponge, symbionts, Thaumarchaetota, ammonia-oxidizers, metaproteome |
Contact List
Stephanie Markert |
contact affiliation | Universität Greifswald Institut für Pharmazie, AG Pharmazeutische Biotechnologie Greifswald, Germany |
contact email | stephanie.markert@uni-greifswald.de |
lab head | |
Stephanie Markert |
contact affiliation | University of Greifswald |
contact email | stephanie.markert@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012484
- Label: PRIDE project
- Name: Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta