PXD012478

PXD012478 is an original dataset announced via ProteomeXchange.

Title	Targets of anti-trophoblast antibodies
Description	Background: Our former study demonstrated that anti-trophoblast antibodies (ATAB) are more prevalent in the sera of patients with unexplained recurrent miscarriages (uRM), and the detection of ATAB is restricted to binding to JEG-3 cells and the quantification by flow cytometry. We further proved that the ATAB-positive sera decrease the production of β-hCG and progesterone in the same cell model. The specific antigenic epitopes of ATAB are still unclear, therefore our aim was to identify one specific target of ATAB in uRM patients. Methods: The possible targets of ATAB were identified by western blots and mass spectrometry, and α-Enolase (ENO1) was further confirmed by a competition assay. The level of anti-ENO1 antibodies in the sera and the production of β-hCG and progesterone were evaluated with enzyme-linked immunosorbent assay (ELISA). Additionally, the expression of ENO1 in the first trimester placenta was analyzed with immunohistochemistry and triple fluorescence staining in uRM patients and healthy controls. Findings: ENO1 was identified to be a prominent target of ATAB and anti-ENO1 antibodies can decrease the secretion of β-hCG and progesterone. The titer of anti-ENO1 antibodies was increased in the sera of ATAB-positive patients compared to ATAB-negative patients. Overexpression of ENO1 and co-expression of ENO1 and β-arrestin was found in the extra villous trophoblasts of uRM patients in the first trimester placenta. Interpretation: Anti-ENO1 antibody in the sera may be a novel autoimmune biomarker of uRM. Decreasing anti-ENO1 antibodies or inhibiting ENO1 expression might be a potential therapeutic treatment for uRM patients.
HostingRepository	PRIDE
AnnounceDate	2019-03-05
AnnouncementXML	Submission_2019-03-05_07:25:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Fröhlich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-23 04:04:20	ID requested
⏵ 1	2019-03-05 07:25:36	announced

Ye Y, Kuhn C, K, ö, sters M, Arnold GJ, Ishikawa-Ankerhold H, Schulz C, Rogenhofer N, Thaler CJ, Mahner S, Fr, ö, hlich T, Jeschke U, von Sch, ö, nfeldt V, -enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages. EBioMedicine, 41():610-622(2019) [pubmed]

curator keyword: Biological

submitter keyword: Unexplained recurrent miscarriages / anti-trophoblast antibodies / JEG-3 cells / anti-α-Enolase antibodies

Thomas Fröhlich
contact affiliation	Laboratory for Functional Genome Analysis LAFUGA Gene Center Ludwig-Maximilians-University 81377 Munich Germany
contact email	frohlich@genzentrum.lmu.de
lab head
Thomas Fröhlich
contact affiliation	Laboratory for Functional Genome Analysis Gene Center Munich Ludwig-Maximilians-Universität Munich (LMU) Feodor-Lynen-Str. 25 81377 Munich Germany
contact email	frohlich@genzentrum.lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012478
     1. Label: PRIDE project
     2. Name: Targets of anti-trophoblast antibodies