PXD012474

PXD012474 is an original dataset announced via ProteomeXchange.

Title	Alternative Splicing of Fibronectin Regulates Composition of the Inflamed Arterial Wall
Description	Exposure of the arterial endothelium to low and disturbed flow is a risk factor for the erosion and rupture of atherosclerotic plaques and aneurysms. Circulating and locally produced proteins are known to contribute to an altered matrix composition at the site of lesions, and to contribute to inflammatory processes within the lesions by altering the sub-endothelial matrix. We have previously shown that immune-cell regulated alternative splicing of Fibronectin (FN) protects against flow-induced hemorrhage. Here, we perform quantitative proteomic analysis of enriched extracellular matrix preparations from murine carotid arteries exposed to low and disturbed flow in vivo and examine serum derived and endothelial cell contributions to the sub-endothelial matrix in vitro. Our results reveal the extent of the dynamic alterations in extracellular matrix composition in the acute response to low and disturbed flow, and show how changes in the splicing of FN, a common response in vascular inflammation and remodeling, affects matrix composition.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:22:38.732.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012474
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Richard Schiavoni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; TMT6plex; Oxidation; Carbamyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-23 02:18:16	ID requested
⏵ 1	2024-10-07 10:22:39	announced

10.6019/PXD012474;

10.1161/atvbaha.120.314013;

Murphy PA, Jailkhani N, Nicholas SA, Del Rosario AM, Balsbaugh JL, Begum S, Kimble A, Hynes RO, Alternative Splicing of FN (Fibronectin) Regulates the Composition of the Arterial Wall Under Low Flow. Arterioscler Thromb Vasc Biol, 41(1):e18-e32(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: Fibronectin

Richard P Schiavoni
contact affiliation	Massachusetts Institute of Technology
contact email	rpschiav@mit.edu
lab head
Richard Schiavoni
contact affiliation	MIT
contact email	rpschiav@mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012474
     1. Label: PRIDE project
     2. Name: Alternative Splicing of Fibronectin Regulates Composition of the Inflamed Arterial Wall