PXD012448

PXD012448 is an original dataset announced via ProteomeXchange.

Title	The Human Gut Firmicute Roseburia intestinalis is a Primary Degrader of Dietary β-Mannans
Description	β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:15:11.081.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Roseburia intestinalis L1-82; NCBI TaxID: 536231;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-21 06:44:13	ID requested
1	2019-02-21 07:09:38	announced
2	2019-02-27 02:18:48	announced	Updated publication reference for PubMed record(s): 30796211.
⏵ 3	2024-10-22 04:15:12	announced	2024-10-22: Updated project metadata.

La Rosa SL, Leth ML, Michalak L, Hansen ME, Pudlo NA, Glowacki R, Pereira G, Workman CT, Arntzen M Ø, Pope PB, Martens EC, Hachem MA, Westereng B, -mannans. Nat Commun, 10(1):905(2019) [pubmed]

10.1038/s41467-019-08812-y;

curator keyword: Biological

submitter keyword: Roseburia intestinalis,Q-Exactive

Bjørge Westereng
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway
contact email	bjorge.westereng@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012448

PRIDE project URI

• PRIDE
  1. PXD012448
     1. Label: PRIDE project
     2. Name: The Human Gut Firmicute Roseburia intestinalis is a Primary Degrader of Dietary β-Mannans