PXD012441

PXD012441 is an original dataset announced via ProteomeXchange.

Title	MSec interactome
Description	Tunneling NanoTubes (TNTs) are membrane conduits that mediate long distance intercellular crosstalk in several organisms and play vital roles during development, pathogenic transmission and cancer metastasis. However, the molecular mechanisms of TNT formation and function remain poorly understood. The protein MSec is essential for TNT formation in multiple cell types. We identified a novel interaction of MSec with the endoplasmic reticulum chaperone ERp29. ERp29 depletion in mammalian cells led to a significant reduction in TNT formation, while its over-expression induced TNT formation, but strictly in an MSec-dependent manner. ERp29 stabilized MSec protein levels but not its mRNA levels, and the chaperone activity of ERp29 was required for maintaining MSec protein stability. Our study implicates MSec as a new target of ERp29 and reveals an indispensable role for the endoplasmic reticulum in the biogenesis of TNTs, thus suggesting new modalities for regulating TNT numbers.
HostingRepository	PRIDE
AnnounceDate	2019-03-15
AnnouncementXML	Submission_2019-03-21_09:48:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sivaram Mylavarapu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-21 06:02:02	ID requested
1	2019-03-15 00:33:32	announced
⏵ 2	2019-03-21 09:48:36	announced	Updated publication reference for PubMed record(s): 30877198.

Pergu R, Dagar S, Kumar H, Kumar R, Bhattacharya J, Mylavarapu SVS, The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec. J Biol Chem, 294(18):7177-7193(2019) [pubmed]

curator keyword: Biological

submitter keyword: Human cellular interactome, SBP affinity purification, ESI-LC-MS

Sivaram V S Mylavarapu
contact affiliation	Laboratory of Cellular Dynamics, Regional Centre for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone Faridabad-Gurgaon Expressway, Faridabad Haryana 121001, India.
contact email	sivaram@rcb.res.in
lab head
Sivaram Mylavarapu
contact affiliation	Regional Centre for Biotechnology, Faridabad, India
contact email	sivaram@rcb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012441

PRIDE project URI

• PRIDE
  1. PXD012441
     1. Label: PRIDE project
     2. Name: MSec interactome