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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-03-15
  • AnnouncementXML: Submission_2019-03-21_09:48:35.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Sivaram Mylavarapu
  • Title: MSec interactome
  • Description: Tunneling NanoTubes (TNTs) are membrane conduits that mediate long distance intercellular crosstalk in several organisms and play vital roles during development, pathogenic transmission and cancer metastasis. However, the molecular mechanisms of TNT formation and function remain poorly understood. The protein MSec is essential for TNT formation in multiple cell types. We identified a novel interaction of MSec with the endoplasmic reticulum chaperone ERp29. ERp29 depletion in mammalian cells led to a significant reduction in TNT formation, while its over-expression induced TNT formation, but strictly in an MSec-dependent manner. ERp29 stabilized MSec protein levels but not its mRNA levels, and the chaperone activity of ERp29 was required for maintaining MSec protein stability. Our study implicates MSec as a new target of ERp29 and reveals an indispensable role for the endoplasmic reticulum in the biogenesis of TNTs, thus suggesting new modalities for regulating TNT numbers.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: TripleTOF 5600

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-01-21 06:02:02ID requested
12019-03-15 00:33:32announced
22019-03-21 09:48:36announcedUpdated publication reference for PubMed record(s): 30877198.

Publication List

  1. Pergu R, Dagar S, Kumar H, Kumar R, Bhattacharya J, Mylavarapu SVS, The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec. J Biol Chem, 294(18):7177-7193(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Human cellular interactome, SBP affinity purification, ESI-LC-MS

Contact List

    Sivaram V S Mylavarapu
    • contact affiliation: Laboratory of Cellular Dynamics, Regional Centre for Biotechnology, NCR Biotech Science Cluster, 3rd Milestone Faridabad-Gurgaon Expressway, Faridabad Haryana 121001, India.
    • contact email: sivaram@rcb.res.in
    • lab head:
    Sivaram Mylavarapu
    • contact affiliation: Regional Centre for Biotechnology, Faridabad, India
    • contact email: sivaram@rcb.res.in
    • dataset submitter:

Full Dataset Link List

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