PXD012439

PXD012439 is an original dataset announced via ProteomeXchange.

Title	Host-symbiont interactions in the chemosynthetic Riftia model symbiosis
Description	The deep-sea tubeworm Riftia pachyptila is a model system for a mutualistic association: The adult worm has no digestive system, but completely relies on one phylotype of endosymbiotic chemosynthetic bacteria for nutrition. The bacteria, in turn, are provisioned by the host. Metabolism and physiology of this symbiosis, particularly of the uncultured symbiont, have been subject to various studies. Yet, how both partners interact on the molecular level remains largely unknown. To study these host-symbiont interactions in detail, we sequenced the R. pachyptila host transcriptome de novo, and conducted comprehensive metaproteomic comparisons of symbiont-containing and symbiont-free R. pachyptila tissues under energy-rich and energy-limiting conditions. Our results demonstrate that R. pachyptila invests a considerable part of its proteome to provision the symbionts with inorganic compounds. It acquires symbiont-derived biomass primarily by digesting parts of the symbiont population. The R. pachyptila immune system apparently not only protects the holobiont from pathogens, but is also involved in symbiont population control. The symbiont expresses a repertoire of proteins dedicated to communication with the host, including eukaryote-like proteins that may counteract phagocytosis. During energy limitation, i.e., when reduced sulfur compounds are lacking, the host apparently increases symbiont digestion. We show here an intricate network of interaction pathways that shapes the R. pachyptila holobiont. Together with the metabolic flexibility of the association under varying energy conditions, this probably forms the basis for the success of this tight association under the highly challenging deep-sea conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:28.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tjorven Hinzke
SpeciesList	scientific name: Candidatus Endoriftia persephone; NCBI TaxID: 393765; scientific name: Riftia pachyptila; NCBI TaxID: 6426;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-21 02:50:56	ID requested
1	2019-11-25 03:26:36	announced
2	2020-01-14 07:17:04	announced	2020-01-14: Updated publication reference for PubMed record(s): 31848270.
⏵ 3	2024-10-22 04:52:29	announced	2024-10-22: Updated project metadata.

10.1128/mBio.02243-19;

Hinzke T, Kleiner M, Breusing C, Felbeck H, H, ä, sler R, Sievert SM, Schl, ü, ter R, Rosenstiel P, Reusch TBH, Schweder T, Markert S, Symbiosis. mBio, 10(6):(2019) [pubmed]

curator keyword: Biological

submitter keyword: mutualism,Metaproteomics, deep sea, hydrothermal vents, holobiont, mutualistic association

Manuel Kleiner
contact affiliation	Department of Geoscience, University of Calgary, Calgary, AB, Canada; Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA
contact email	manuel_kleiner@ncsu.edu
lab head
Tjorven Hinzke
contact affiliation	University of Greifswald, Greifswald Intitute of Marine Biotechnology
contact email	tjorven.hinzke@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012439

PRIDE project URI

• PRIDE
  1. PXD012439
     1. Label: PRIDE project
     2. Name: Host-symbiont interactions in the chemosynthetic Riftia model symbiosis