PXD012431

PXD012431 is an original dataset announced via ProteomeXchange.

Title	High-throughput mass spectrometry and bioinformatics analysis of breast cancer proteomic data
Description	The project contains raw and result files from a comparative proteomic analysis of malignant [primary breast tumor (PT) and axillary metastatic lymph nodes (LN)] and non-tumor [contralateral (NCT) and adjacent breast (ANT)] tissues of patients diagnosed with invasive ductal carcinoma. A label-free mass spectrometry was conducted using nano-liquid chromatography coupled to electrospray ionization–mass spectrometry (LC-ESI-MS/MS) followed by functional annotation to reveal differentially expressed proteins and their predicted impacts on pathways and cellular functions in breast cancer. A total of 462 proteins was observed as differentially expressed (DEPs) among the groups of samples analyzed. Ingenuity Pathway Analysis software version 2.3 (QIAGEN Inc.) was employed to identify the most relevant signaling and metabolic pathways, diseases, biological functions and interaction networks affected by the deregulated proteins. Upstream regulator and biomarker analyses were also performed by IPA’s tools. Altogether, our findings revealed differential proteomic profiles that affected the associated and interconnected cancer signaling processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:27.629.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Batista
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-18 07:42:11	ID requested
1	2019-07-15 04:41:10	announced
⏵ 2	2024-10-22 04:52:28	announced	2024-10-22: Updated project metadata.

Gomig THB, Cavalli IJ, Souza RLR, Lucena ACR, Batista M, Machado KC, Marchini FK, Marchi FA, Lima RS, Urban CA, Cavalli LR, Ribeiro EMSF, High-throughput mass spectrometry and bioinformatics analysis of breast cancer proteomic data. Data Brief, 25():104125(2019) [pubmed]

10.1016/j.dib.2019.104125;

curator keyword: Biomedical

submitter keyword: proteomics, contralateral breast tissue, label-free mass spectrometry,Breast cancer, bioinformatics analysis

Enilze Maria de Souza Fonseca Ribeiro
contact affiliation	Genetics Department, Federal University of Parana, Curitiba, Brazil
contact email	enilzeribeiro@gmail.com
lab head
Michel Batista
contact affiliation	Fiocruz
contact email	batista.mic@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012431

PRIDE project URI

• PRIDE
  1. PXD012431
     1. Label: PRIDE project
     2. Name: High-throughput mass spectrometry and bioinformatics analysis of breast cancer proteomic data