PXD012426

PXD012426 is an original dataset announced via ProteomeXchange.

Title	Searching for binding partners of the SLX4 577-795 region and assessing the impact of the SLX4 D614G and L618P cancer patient-derived mutations on their association with SLX4
Description	The project was aimed at identifying new binding partners of SLX4 that associate with a region spanning the conserved amphipathic helix of SLX4 and the downstream BTB domain. It was also aimed at assessing which of those partners might have their association with SLX4 abrogated by the cancer patient-derived D614G and L618P SLX4 mutations. The goal was ultimately to determine whether RTEL1 which we had identified as a novel binding partner of SLX4 and that does not bind SLX4 D614G or SLX4 L618P mutant proteins, is the only SLX4 binding partner to be impacted by those mutations. A YFP-SLX4 577-795 wt or mutated fragment produced in Hela Flp-In TRex cells was immunoprecipitated using a GFP-nanobody. YFP-pull downs were washed 5 times with 50 mM Tris-HCl [pH 8.0] buffer elution of the proteins bound to the GFP-nanobody directly in NuPAGE LDS sample buffer (Invitrogen) for 5 min at 95˚C.
HostingRepository	PRIDE
AnnounceDate	2020-03-05
AnnouncementXML	Submission_2020-06-12_01:41:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-18 03:12:35	ID requested
1	2020-03-05 00:25:24	announced
⏵ 2	2020-06-12 01:41:56	announced	2020-06-12: Updated publication reference for PubMed record(s): 32398829.

Takedachi A, Despras E, Scaglione S, Gu, é, rois R, Guervilly JH, Blin M, Audebert S, Camoin L, Hasanova Z, Schertzer M, Guille A, Churikov D, Callebaut I, Naim V, Chaffanet M, Borg JP, Bertucci F, Revy P, Birnbaum D, Londo, ñ, o-Vallejo A, Kannouche PL, Gaillard PHL, SLX4 interacts with RTEL1 to prevent transcription-mediated DNA replication perturbations. Nat Struct Mol Biol, 27(5):438-449(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: SLX4 scaffold, Cancer associated mutations, protein-protein binding domain, interactome

MarseilleProteomics CRCM and Gaillard PH Lab
contact affiliation	Inserm UMR1068, CNRS UMR7258, Aix Marseille université U105, Institut Paoli Calmettes, CRCM
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012426

PRIDE project URI

• PRIDE
  1. PXD012426
     1. Label: PRIDE project
     2. Name: Searching for binding partners of the SLX4 577-795 region and assessing the impact of the SLX4 D614G and L618P cancer patient-derived mutations on their association with SLX4