PXD012419

PXD012419 is an original dataset announced via ProteomeXchange.

Title	Pratylenchus penetrans PME - Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
Description	Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, showing the presence of four introns that excluded a potential bacterial contamination. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated for early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in N. benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:24.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cláudia Vicente
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-17 08:01:08	ID requested
1	2019-02-28 04:39:25	announced
⏵ 2	2024-10-22 04:52:32	announced	2024-10-22: Updated project metadata.

10.1371/journal.pone.0212540;

Vicente CSL, Nemchinov LG, Mota M, Eisenback JD, Kamo K, Vieira P, Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans. PLoS One, 14(2):e0212540(2019) [pubmed]

curator keyword: Biological

submitter keyword: PME,Plant parasitic nematode, LC-MS/MS

Paulo Vieira and Len Nemchinov
contact affiliation	Molecular Plant Pathology Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, United States of America
contact email	paulo.vieira@ars.usda.gov
lab head
Cláudia Vicente
contact affiliation	ICAAM - University of Evora
contact email	cvicente@uevora.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012419

PRIDE project URI

• PRIDE
  1. PXD012419
     1. Label: PRIDE project
     2. Name: Pratylenchus penetrans PME - Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans