PXD012391

PXD012391 is an original dataset announced via ProteomeXchange.

Title	Algal Protein Kinase, Triacylglycerol Accumulation Regulator1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen Imbalanced Conditions
Description	Nutrient-deprived microalgae accumulate triacylglycerol (TAG) in lipid droplets. A Dual-specificity tyrosine phosphorylation-regulated kinase, TAG-accumulation-regulator-1 (TAR1) has been shown to be required for acetate-dependent TAG accumulation and degradation of chlorophyll and photosynthesis-related proteins in photomixotrophic nitrogen (N)-deficient conditions (Kajikawa et al. 2015). However, this previous report only examined the particular condition. Here, we report that under photoautotrophic N-deficient conditions, tar1-1 cells, with a mutation in the TAR1 gene, accumulated higher levels of TAG and starch, and maintained higher levels of cell viability and lower levels of H2O2 generation compared to wild-type (WT) cells. Transcriptomic analyses suggest that genes involved in the scavenging of reactive oxygen species, such as APX1, GPX5, PTOX1, TRX7 are not repressed in tar1-1cells. In contrast, mating efficiency and mRNA levels for key regulators in gametogenesis, MID, MTD and FUS, were suppressed in tar1-1 cells. Among the 426 TAR1-dependent phosphoproteins detected by phosphoproteomic analysis, several protein kinases and enzymes related to N assimilation and carbon metabolism are of particular interest. Characterization of these putative downstream factors may elucidate the molecular pathway whereby TAR1 mediates cellular propagation and carbon- and nitrogen- metabolism under C/N imbalance stress conditions.
HostingRepository	jPOST
AnnounceDate	2019-07-22
AnnouncementXML	Submission_2022-09-18_02:34:50.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naoyuki Sugiyama
SpeciesList	scientific name: Chlamydomonas; NCBI TaxID: 3052;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; alpha-amino dimethylated residue; N6,N6-dimethyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 17:24:26	ID requested
1	2019-07-21 17:32:38	announced
2	2021-02-04 17:16:47	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 02:34:50	announced	2022-09-18: Updated FTP location.

Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H, Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions. Plant Cell Physiol, 60(4):916-930(2019) [pubmed]

submitter keyword: C/N balance, Chlamydomonas, dual-specificity tyrosine-phosphorylationregulated kinase, gametogenesis, hydrogen peroxide, phosphoproteome

Hideya Fukuzawa
lab head
Naoyuki Sugiyama
contact affiliation	Kyoto University
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000544/