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PXD012377

PXD012377 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA folded conformation of MukBEF and Cohesin
DescriptionStructural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the underlying conformational changes are largely unclear. Using structural biology, mass spectrometry and cross-linking, we investigated the architecture of two evolutionarily distant SMC-kleisin complexes: proteobacterial MukBEF and eukaryotic cohesin. We show that both contain a dynamic coiled-coil discontinuity, the elbow, near the middle of their arms that permits a folded conformation. Bending at the elbow brings into proximity the hinge dimerization domain and the head/kleisin module, situated at opposite ends of the arms. Our findings favor SMC activity models that include a large conformational change in the arms, such as a relative movement between DNA binding sites during DNA loading and translocation
HostingRepositoryPRIDE
AnnounceDate2019-03-01
AnnouncementXMLSubmission_2019-05-27_01:40:49.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancis O'Reilly
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListcarbamoylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-16 06:25:37ID requested
12019-03-01 00:07:50announced
22019-05-27 01:40:50announcedUpdated publication reference for PubMed record(s): 30833788.
Publication List
B, ü, rmann F, Lee BG, Than T, Sinn L, O'Reilly FJ, Yatskevich S, Rappsilber J, Hu B, Nasmyth K, L, ö, we J, A folded conformation of MukBEF and cohesin. Nat Struct Mol Biol, 26(3):227-236(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: crosslinking, MukBEF, cohesin, condensin, kleisin, Structural Maintenance of Chromosomes, SMC proteins, DNA translocation, loop extrusion
Contact List
Juri Rappsilber
contact affiliationBioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK.
contact emailjuri.rappsilber@ed.ac.uk
lab head
Francis O'Reilly
contact affiliationTU Berlin
contact emailoreilly@tu-berlin.de
dataset submitter
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Dataset FTP location
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