PXD012371

PXD012371 is an original dataset announced via ProteomeXchange.

Title	Bald thigh syndrome in sighthounds
Description	Bald thigh syndrome is a common hair loss disorder in sighthounds. The overall goal of our study was to identify the cause of bald thigh syndrome and the pathological changes associated with it. We approached this aim by comparing skin biopsies and hair shafts of affected and control dogs microscopically as well as by applying high-throughput technologies such as genomics, transcriptomics and proteomics. While the histology is rather unspecific in most cases, trichogram analysis and scanning electron microscopy revealed severe structural abnormalities in hair shafts of affected dogs. This finding is supported by the results of the transcriptomic and proteomic profiling of skin biopsies and hair shafts, respectively, where genes and proteins important for differentiation of the inner root sheath and the assembly of a proper hair shaft were downregulated. Transcriptome profiling of skin biopsies revealed a downregulation of genes encoding 23 hair shaft keratins and 51 keratin associated proteins, as well as desmosomal cadherins and several actors of the BMP signaling pathway, which is important for hair shaft differentiation. To identify differentially expressed proteins in structural abnormal hair shafts, we performed nLC-MS/MS- based proteomic analysis of fractured hair shafts of four dogs with bald thigh syndrome (Greyhounds, n=3, Whippet, n=1) and intact telogen hair shafts of four control dogs (Greyhounds, n=3, Whippet= n=1) plucked on the thighs. Decreased expression of keratin 71 and desmocollin 2 on the mRNA level in skin biopsies corresponded with a reduced protein expression in the hair shafts of affected dogs.
HostingRepository	PRIDE
AnnounceDate	2019-11-13
AnnouncementXML	Submission_2019-11-12_17:31:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 01:06:01	ID requested
⏵ 1	2019-11-12 17:32:01	announced

Brunner MAT, R, ü, fenacht S, Bauer A, Erpel S, Buchs N, Braga-Lagache S, Heller M, Leeb T, Jagannathan V, Wiener DJ, Welle MM, Bald thigh syndrome in sighthounds-Revisiting the cause of a well-known disease. PLoS One, 14(2):e0212645(2019) [pubmed]

curator keyword: Biological

submitter keyword: greyhound dog, bald thigh syndrome, hair, proteome

Monika Maria Welle
contact affiliation	Institut für Tierpathologie (ITPA) Vetsuisse University of Bern Länggassstrasse 122 3001 Bern
contact email	monika.welle@vetsuisse.unibe.ch
lab head
Manfred Heller
contact affiliation	University of Bern
contact email	manfred.heller@dbmr.unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012371
     1. Label: PRIDE project
     2. Name: Bald thigh syndrome in sighthounds