PXD012355

PXD012355 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes in the milk of water buffalo (Bubalus bubalis) with intramammary infection by Staphylococcus aureus and by non-aureus staphylococciProteomic changes in the milk of water buffalo (Bubalus bubalis) with intramammary infection by Staphylococcus aureus and by non-aureus staphylococci
Description	The study reports a differential proteomic analysis of the Mediterranean buffalo milk to evaluate the changes induced by Staphylococcus spp. during a subclinical intramammary infection (IMI). A number of 12 quarter milk samples, 6 of which with somatic cell count (SCC) < 50,000 cells/mL and culture-negative, and the other 6 with SCC ˃ 3,000,000 cells/mL and culture-positive to Staphylococcus aureus (SAU, n=3), SAU, or non-aureus staphylococci (NAS, n=3) was selected. Samples were analyzed using a shotgun proteomics approach, based on filter-aided sample preparation (FASP) followed by LC-MS/MS and label-free analysis. Here, the largest buffalo milk protein dataset described so far was reported. Moreover, the results demonstrated that staphylococcal IMI mostly affected proteins involved in structural functions and in innate immune defense, with changes in their abundance that were generally more intense in SAU than in NAS samples. Further, an increase in the abundance of different cathelicidins was observed, as already reported for other animals with mastitis disease (1,2). (1) Addis MF, Pisanu S, Marogna G, Cubeddu T, Pagnozzi D, Cacciotto C, et al. Production and release of antimicrobial and immune defense proteins by mammary epithelial cells following Streptococcus uberis infection of sheep. Infect Immun. 2013;81: 3182–3197. (2) Addis MF, Tedde V, Dore S, Pisanu S, Puggioni GMG, Roggio AM, et al. Evaluation of milk cathelicidin for detection of dairy sheep mastitis. J Dairy Sci. Elsevier; 2016;99: 6446–6456. In conclusion, our results provide the first in depth characterization of buffalo milk proteins, describe the changes induced by SAU and NAS subclinical intramammary infection and suggest indications to reveal subclinical staphylococcal mastitis in buffalo by the milk proteome investigation.
HostingRepository	PRIDE
AnnounceDate	2019-10-22
AnnouncementXML	Submission_2019-11-06_00:33:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salvatore Pisanu
SpeciesList	scientific name: Bubalus bubalis; NCBI TaxID: 89462;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-15 07:37:00	ID requested
1	2019-10-21 22:02:05	announced
⏵ 2	2019-11-06 00:33:59	announced	2019-11-06: Updated publication reference for PubMed record(s): 31676851.

Pisanu S, Cacciotto C, Pagnozzi D, Puggioni GMG, Uzzau S, Ciaramella P, Guccione J, Penati M, Pollera C, Moroni P, Bronzo V, Addis MF, Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci. Sci Rep, 9(1):15850(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Buffalo milk, mastitis, Label-free proteomics, LC-MS/MS

Daniela Pagnozzi
contact affiliation	Porto Conte Ricerche, Alghero, Sardinia, Italy
contact email	pagnozzi@portocontericerche.it
lab head
Salvatore Pisanu
contact affiliation	Porto Conte Ricerche, Alghero, Sardinia, Italy.
contact email	pisanu@portocontericerche.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012355
     1. Label: PRIDE project
     2. Name: Proteomic changes in the milk of water buffalo (Bubalus bubalis) with intramammary infection by Staphylococcus aureus and by non-aureus staphylococciProteomic changes in the milk of water buffalo (Bubalus bubalis) with intramammary infection by Staphylococcus aureus and by non-aureus staphylococci