PXD012347

PXD012347 is an original dataset announced via ProteomeXchange.

Title	Natural variation of H3K27me3 determines genes important for cell fate in the unicellular eukaryotic model Phaeodactylum tricornutum
Description	In multicellular organisms, tri-methylation of lysine 27 of histone H3 (H3K27me3) was shown to be deposited by Polycomb Repressive Complex 2 (PRC2) to establish and maintain silencing, critical for cell fate and developmentally regulated processes. PRC2 complex is absent in both yeast Saccharomyces cerevisiae and S. pombe which initially suggested that PRC2 arose with the emergence of multicellularity. However, its discovery in several microalgae questions its role in this important class of organisms. Here, we show using mutants of the homologue of catalytic unit of PRC2, enhancer of zeste E(z) in the model diatom P. tricornutum (Pt), which presents different morphotypes (fusiform, triradiate, oval and cruciform) that Pt E(z) is responsible of di and tri-methylation of lysine 27. Indeed, H3K27me3 depletion causes the distortion of the morphology providing evidence for a role of H3K27me3 in cell differentiation in unicellular species. H3K27me3 genome wide profiling in fusiform and triradiate further revealed genes important for cell identity. These results suggest a role of PRC2 and its associated mark in cell fate in unicellular species and their ancestral function in a broad evolutionary context.
HostingRepository	PRIDE
AnnounceDate	2020-12-07
AnnouncementXML	Submission_2020-12-07_03:53:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Arras
SpeciesList	scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationList	trimethylated residue; dimethylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-15 01:50:48	ID requested
⏵ 1	2020-12-07 03:53:34	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Phaeodactylum tricornutum, H3K27me3, H3K27me2, enhancer of zeste

Damarys Loew
contact affiliation	Laboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University
contact email	Damarys.Loew@curie.fr
lab head
Guillaume Arras
contact affiliation	Institut Curie
contact email	guillaume.arras@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD012347

PRIDE project URI

• PRIDE
  1. PXD012347
     1. Label: PRIDE project
     2. Name: Natural variation of H3K27me3 determines genes important for cell fate in the unicellular eukaryotic model Phaeodactylum tricornutum