Bermudagrass is an important warm-season turfgrass species with both erect growing shoots and prostrate growing stolons, however, the mechanism how bermudagrass shoots and stolons form and maintain their unique geotropic growth modes are still unclear. In this study, we compared the proteome of the internode section of shoots and stolons at the same developmental stage in bermudagrass cultivar Yangjiang. The results indicated that 376 protein species were differentially accumulated in the two types of stems.