PXD012322

PXD012322 is an original dataset announced via ProteomeXchange.

Title	Matrisome properties of scaffolds direct fibroblasts in idiopathic pulmonary fibrosis
Description	In this study we have focused on the biomechanical properties of scaffolds (decellularized lung tissue) derived from healthy individuals and IPF patients. The longitudinal cellular response of scaffold repopulation with healthy fibroblasts has been quantified using SILAC-MS. Increased scaffold density and stiffness along with differential expressions of proteins clearly separated and defined ECM proteins descriptive for IPF respective healthy scaffolds. Our study aim to understand the role of the ECM in the development of IPF. We have used proteomics to define intrinsic scaffold ECM proteins descriptive for healthy respective IPF scaffolds. To evaluate whether these mediators directs or rejects profibrotic responses fibroblasts repopulated on healthy and IPF derived scaffolds were cultured in heavy labeled medie (SILAC) to differentiate between preexisting scaffold proteins and newly synthesized proteins. The spatial distribution of unique ECM proteins characteristic for healthy fibroblasts on IPF scaffolds were verified with histological staining.
HostingRepository	PRIDE
AnnounceDate	2019-08-27
AnnouncementXML	Submission_2019-08-27_05:44:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Åhrman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-14 06:03:02	ID requested
⏵ 1	2019-08-27 05:44:33	announced

Elowsson Rendin L, L, ö, fdahl A, Å, hrman E, M, ü, ller C, Notermans T, Michalikov, á B, Rosmark O, Zhou XH, Dellgren G, Silverborn M, Bjermer L, Malmstr, ö, m A, Larsson-Callerfelt AK, Isaksson H, Malmstr, ö, m J, Westergren-Thorsson G, Matrisome Properties of Scaffolds Direct Fibroblasts in Idiopathic Pulmonary Fibrosis. Int J Mol Sci, 20(16):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Lung tissue, scaffold, IPF, repopulation, SILAC-MS

Johan Malmström
contact affiliation	Infection Medicine Proteomics BMC D13 Klinikgatan 28 22241 Lund Sweden
contact email	johan.malmstrom@med.lu.se
lab head
Emma Åhrman
contact affiliation	Lund University
contact email	emma.ahrman@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD012322

PRIDE project URI

• PRIDE
  1. PXD012322
     1. Label: PRIDE project
     2. Name: Matrisome properties of scaffolds direct fibroblasts in idiopathic pulmonary fibrosis