PXD012311

PXD012311 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of Chlamydomonas reinhardtii in photoautotrophic nitrogen-deficient conditions
Description	Nutrient-deprived microalgae accumulate triacylglycerol (TAG) in lipid droplets. A dual-specificity tyrosine phosphorylation-regulated kinase, TAG accumulation regulator 1 (TAR1) has been shown to be required for acetate-dependent TAG accumulation and the degradation of chlorophyll and photosynthesis-related proteins in photomixotrophic nitrogen (N)-deficient conditions (Kajikawa et al. 2015). However, this previous report only examined particular condition. Here, we report that in photoautotrophic N-deficient conditions, tar1-1 cells, with a mutation in the TAR1 gene, maintained higher levels of cell viability and lower levels of hydrogen peroxide generation and accumulated higher levels of TAG and starch compared with those of wild-type (WT) cells with bubbling of air containing 5% carbon dioxide. Transcriptomic analyses suggested that genes involved in the scavenging of reactive oxygen species are not repressed in tar1-1 cells. In contrast, the mating efficiency and mRNA levels of key regulatory genes for gametogenesis, MID, MTD, and FUS, were suppressed in tar1-1 cells. Among the TAR1-dependent phosphopeptides deduced by phosphoproteomic analysis, protein kinases and enzymes related to N assimilation and carbon (C) metabolism are of particular interest. Characterization of these putative downstream factors may elucidate the molecular pathway whereby TAR1 mediates cellular propagation and C and N metabolism in C/N-imbalanced stress conditions.
HostingRepository	jPOST
AnnounceDate	2019-01-14
AnnouncementXML	Submission_2022-09-18_02:56:41.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hirofumi Nakagami
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-11 08:24:48	ID requested
1	2019-01-14 01:08:45	announced
2	2020-01-14 07:09:46	announced	2020-01-15: Updated PubMed.
3	2021-02-04 16:49:12	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:56:42	announced	2022-09-18: Updated FTP location.

Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H, Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions. Plant Cell Physiol, 60(4):916-930(2019) [pubmed]

submitter keyword: Chlamydomonas reinhardtii, Triacylglycerol accumulation regulator 1 (TAR1), Photoautotrophic nitrogen-deficient conditions, Phosphoproteomics

Hirofumi Nakagami
lab head
Hirofumi Nakagami
contact affiliation	Max Planck Institute for Plant Breeding Research
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000542/