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PXD012311

PXD012311 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics of Chlamydomonas reinhardtii in photoautotrophic nitrogen-deficient conditions
DescriptionNutrient-deprived microalgae accumulate triacylglycerol (TAG) in lipid droplets. A dual-specificity tyrosine phosphorylation-regulated kinase, TAG accumulation regulator 1 (TAR1) has been shown to be required for acetate-dependent TAG accumulation and the degradation of chlorophyll and photosynthesis-related proteins in photomixotrophic nitrogen (N)-deficient conditions (Kajikawa et al. 2015). However, this previous report only examined particular condition. Here, we report that in photoautotrophic N-deficient conditions, tar1-1 cells, with a mutation in the TAR1 gene, maintained higher levels of cell viability and lower levels of hydrogen peroxide generation and accumulated higher levels of TAG and starch compared with those of wild-type (WT) cells with bubbling of air containing 5% carbon dioxide. Transcriptomic analyses suggested that genes involved in the scavenging of reactive oxygen species are not repressed in tar1-1 cells. In contrast, the mating efficiency and mRNA levels of key regulatory genes for gametogenesis, MID, MTD, and FUS, were suppressed in tar1-1 cells. Among the TAR1-dependent phosphopeptides deduced by phosphoproteomic analysis, protein kinases and enzymes related to N assimilation and carbon (C) metabolism are of particular interest. Characterization of these putative downstream factors may elucidate the molecular pathway whereby TAR1 mediates cellular propagation and C and N metabolism in C/N-imbalanced stress conditions.
HostingRepositoryjPOST
AnnounceDate2019-01-14
AnnouncementXMLSubmission_2022-09-18_02:56:41.901.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHirofumi Nakagami
SpeciesList scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-11 08:24:48ID requested
12019-01-14 01:08:45announced
22020-01-14 07:09:46announced2020-01-15: Updated PubMed.
32021-02-04 16:49:12announced2021-02-04: Updated FTP location.
42022-09-18 02:56:42announced2022-09-18: Updated FTP location.
Publication List
Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H, Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions. Plant Cell Physiol, 60(4):916-930(2019) [pubmed]
Keyword List
submitter keyword: Chlamydomonas reinhardtii, Triacylglycerol accumulation regulator 1 (TAR1), Photoautotrophic nitrogen-deficient conditions, Phosphoproteomics
Contact List
Hirofumi Nakagami
lab head
Hirofumi Nakagami
contact affiliationMax Planck Institute for Plant Breeding Research
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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