PXD012305

PXD012305 is an original dataset announced via ProteomeXchange.

Title	NPM and NPM-MLF1 interact with chromatin remodeling complexes and influence their recruitment to specific genes
Description	Nucleophosmin (NPM1) is either frequently mutated or subjected to chromosomal translocation in acute myeloid leukemia (AML). NPM protein is primarily located in the nucleus, but the recurrent NPMc+ mutation is characterized by cytoplasmic localization and leukemogenic properties. Similarly, the NPM-MLF1 translocation product favors the partial cytoplasmic retention of NPM. Regardless of their common cellular distribution, NPM-MLF1 malignancies engender different effects on hematopoiesis compared to NPMc+ counterparts, highlighting possible aberrant nuclear function(s) of NPM in NPMc+ and NPM-MLF1 AML. We performed a proteomics analysis and found that NPM and NPM-MLF1 interact with chromatin remodeling complexes of the ISWI family, as well as with NuRD and P/BAF complexes. Accordingly, NPM and NPM-MLF1 are recruited to transcriptionally active or repressed genes along with NuRD and P/BAF elements. Although the overall gene expression program in NPM knockdown cells is similar to that resulting from NPMc+ and is consistent with loss of nuclear function of NPM, NPM-MLF1 expression differentially altered gene transcription regulated by NPM. The abnormal gene regulation imposed by NPM-MLF1 is characterized by enhanced recruitment of NuRD to gene regulatory regions. Thus, different mechanisms orchestrate the deregulation of NPM function in NPMc+ -versus NPM1-MLF1- associated leukemia.
HostingRepository	PRIDE
AnnounceDate	2019-10-18
AnnouncementXML	Submission_2019-11-12_02:10:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eric Milot
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-11 04:51:30	ID requested
1	2019-10-17 23:39:11	announced
⏵ 2	2019-11-12 02:10:44	announced	2019-11-12: Updated publication reference for PubMed record(s): 31675375.

Darracq A, Pak H, Bourgoin V, Zmiri F, Dellaire G, Affar EB, Milot E, NPM and NPM-MLF1 interact with chromatin remodeling complexes and influence their recruitment to specific genes. PLoS Genet, 15(11):e1008463(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: NPM, NPM-MLF1, leukemia, chromatin remodeling complexes, proteomic, epigenetic

Dr Milot Eric
contact affiliation	(1) Maisonneuve Rosemont Hospital Research Center, CIUSSS Est de l’Île de Montréal, Montreal, QC, Canada (2) Department of Medicine, University of Montreal, Montréal, QC, Canada
contact email	e.milot.1@umontreal.ca
lab head
Eric Milot
contact affiliation	Université de Montréal
contact email	emilot.hmr@ssss.gouv.qc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD012305

PRIDE project URI

• PRIDE
  1. PXD012305
     1. Label: PRIDE project
     2. Name: NPM and NPM-MLF1 interact with chromatin remodeling complexes and influence their recruitment to specific genes