PXD012303

PXD012303 is an original dataset announced via ProteomeXchange.

Title	SILAC analysis of long bone osteoblasts isolated from Tgif1+/+ and Tgif1-/- mice
Description	Eric Hesse 9 Jan 2019, 17:34 (15 hours ago) to me, Hartmut Lieber Marcel, unten ist das abstract kopiert, reicht das? Lg, Eric Abstract Osteoporosis is caused by increased bone resorption and decreased bone formation. Intermittent administration of a fragment of Parathyroid hormone (PTH) activates osteoblast-mediated bone formation and is used in patients with severe osteoporosis. However, the mechanisms by which PTH elicits its anabolic effect are not fully elucidated. Here we show that the absence of the homeodomain protein TG-interacting factor 1 (Tgif1) impairs osteoblast differentiation and activity, leading to a reduced bone formation. Deletion of Tgif1 in osteoblasts and osteocytes decreases bone resorption due to an increased secretion of Semaphorin 3E (Sema3E), an osteoclast-inhibiting factor. Tgif1 is a PTH target gene and PTH treatment failed to increase bone formation and bone mass in Tgif1-deficient mice. Thus, our study identifies Tgif1 as a novel regulator of bone remodeling and an essential component of the PTH anabolic action. These insights contribute to a better understanding of bone metabolism and the anabolic function of PTH.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:08.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcel Kwiatkowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-10 07:48:16	ID requested
1	2019-01-29 00:01:28	announced
⏵ 2	2024-10-22 04:52:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: LC-MS, SILAC, Tgif1,osteoblasts

Eric Hesse
contact affiliation	Molecular Skeletal Biology Laboratory, Department of Trauma, Hand and Reconstructive Surgery, University-Medical Center Hamburg-Eppendorf, Hamburg, Germany.
contact email	e.hesse@uke.de
lab head
Marcel Kwiatkowski
contact affiliation	University of Groningen
contact email	m.d.kwiatkowski@rug.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD012303

PRIDE project URI

• PRIDE
  1. PXD012303
     1. Label: PRIDE project
     2. Name: SILAC analysis of long bone osteoblasts isolated from Tgif1+/+ and Tgif1-/- mice